Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-03T00:57:48.858Z Has data issue: false hasContentIssue false
  • English
  • Français

Taenia crassiceps surface immunoglobulins: parasite- or host-derived?

Published online by Cambridge University Press:  06 April 2009

D. P. McManus  and
S. Lamsam
D. P. McManus
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, Bramston Terrace, Brisbane, Queensland 4006, Australia Department of Pure and Applied Biology, Imperial College of Science, Technology and Medicine, London SW7 2BB, U.K.
S. Lamsam
Affiliation:
Department of Pure and Applied Biology, Imperial College of Science, Technology and Medicine, London SW7 2BB, U.K.
Get access Rights & Permissions [Opens in a new window]

Summary

In common with other taeniid cestodes, host or host-like proteins, especially immunoglobulins, occur on the surface and in the cyst fluid of Taenia crassiceps metacestodes. Here, several approaches have been used to determine the origin of the immunoglobulins present on the tegument. Indirect IFAT showed that IgG was almost totally lost from the surface of bladders after 6 days culture in vitro. There was a rapid reacquisition of immunoglobulins following incubation of the cultured metacestodes with either normal mouse serum or mouse anti-T. crassiceps antiserum. Immunoprecipitation of in vitro translation products and biosynthetically labelled T. crassiceps proteins with a panel of anti-IgG antisera failed to positively identify any molecule with homology to mammalian immunoglobulins. These results suggest strongly that the immunoglobulins located on the surface of T. crassiceps are of host rather than parasite origin. The occurrence of a relatively low abundance receptor in the surface of the bladders, which binds non-specific host immunoglobulin, together with surface-bound specific anti-T. crassiceps antibodies can account for the presence of these host proteins. Freshly obtained bladders and metacestodes cultured in vitro for 6 days were transplanted into naive mice and the survival and development of the resulting parasites compared. In some individual mice there was a decrease in the number and volume of metacestodes and an increase in encapsulated parasites arising from cultured bladders. This was probably not related to the loss of host immunoglobulins from the parasite surface during culture as the reacquisition of these proteins after transplantation is likely to be far more rapid than any immune response could evoke in a naive host.

Keywords

Taenia crassicepsmolecular mimicryIgG‘blocking’ antibodiesin vitro translationbiosynthetic labellingtransplantation
Type
Research Article
Information
Parasitology , Volume 101 , Issue 1 , August 1990 , pp. 127 - 137
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alkarmi, T. O., Alshakarchi, Z. & Behbehani, K. (1988). Echinococcus multilocularis: the non-specific binding of different species of immunoglobulins to alveolar hydatid cysts grown in vivo and in vitro. Parasite Immunology 10, 442–57.CrossRefGoogle ScholarPubMed
Chernin, J. (1977). Common host antigens in laboratory rats infected with metacestodes of Taenia crassiceps. Journal of Helminthology 51, 215–20.CrossRefGoogle ScholarPubMed
Chernin, J. (1982). The nature of antigens common to both the metacestodes of Taenia crassiceps and its laboratory host. Journal of Helminthology 56, 339–414.CrossRefGoogle ScholarPubMed
Chew, M. W. K. (1981). Taenia crassiceps: structure, developmental biology and host-parasite relationship. Ph.D. thesis, University of London.Google Scholar
Chirgwin, J. M., Pryzbla, A. E., MacDonald, R. J. & Rutter, W. J. (1979). Isolation of biologically active RNA from sources enriched in ribonuclease. Biochemistry 18, 5294–8.CrossRefGoogle ScholarPubMed
Craig, P. S. (1988). Surface-associated proteins and host IgG on early and late metacestode stages of Taenia pisiformis. Parasite Immunology 10, 243–54.CrossRefGoogle ScholarPubMed
Damian, R. T. (1964). Molecular mimicry: antigen sharing by parasite and host and its consequences. American Naturalist 98, 129–49.CrossRefGoogle Scholar
Damian, R. T. (1987 a). The exploitation of host immune response by parasites. Journal of Parasitology 73, 1–13.CrossRefGoogle ScholarPubMed
Damian, R. T. (1987 b). Molecular mimicry revisited. Parasitology Today 3, 263–6.CrossRefGoogle ScholarPubMed
Díaz De León, L., Arcos, L. & Willms, K. (1982). The Use of cell-free systems for the characterization of Cysticercus cellulosae antigens. In Cysticercosis: Present State of Knowledge and Perspectives (ed. Flisser, A., Willms, A., Laclette, J. P., Larralde, C., Ridaura, C., & Beltran, F.) pp. 465–77. New York and London: Academic Press.Google Scholar
Flisser, A., Espinoza, B., Tovar, A., Plancarte, A. & Correa, D. (1986). Host-parasite relationship in cysticercosis: immunologic study in different compartments of the host. Veterinary Parasitology 20, 95–102.CrossRefGoogle ScholarPubMed
Hayunga, E. G., Sumner, M. P. & Letonja, T. (1989). Evidence for selective incorporation of host immunoglobulin by strobilocerci of Taenia taeniaeformis. Journal of Parasitology 75, 638–42.CrossRefGoogle ScholarPubMed
Kessler, S. W. (1975). Rapid isolation of antigens from cells with a Staphylococcal protein A-antibody absorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115, 1617–24.CrossRefGoogle Scholar
Kleine-Herzbruch, R. & Geyer, E. (1988). Comparison of the in vitro translation capacity of Taenia crassiceps metacestode in mRNA prepared by the phenol and cesium chloride method. Parasitology Research 74, 469–75.CrossRefGoogle ScholarPubMed
Kwa, B. K. & Liew, F. Y. (1978). Studies on the mechanism of long term survival of Taenia taeniaeformis in rats. Journal of Helminthology 52, 1–6.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Lamsam, S. & McManus, D. P. (1990). Molecular characterization of the surface and cyst fluid components of Taenia crassiceps. Parasitology 101, 115–25.CrossRefGoogle ScholarPubMed
McLaren, D. (1984). Disguise as an evasive stratagem of parasitic organisms. Parasitology 88, 597–611.CrossRefGoogle ScholarPubMed
Rickard, M. J. (1974). Hypothesis for the long term survival of Taenia pisiformis cysticerci in rabbits. Zeitschrift für Parasitenkunde 44, 203–9.CrossRefGoogle Scholar
Siebert, A. E., Jr., Blitz, R. R., Morita, C. T. & Good, A. H. (1981). Taenia crassiceps: serum and surface immunoglobulins in metacestode infections of mice. Experimental Parasitology 51, 418–30.CrossRefGoogle ScholarPubMed
Siebert, A. E. Jr. & Good, A. H. (1979). Taenia crassiceps: effect of normal and immune serum on metacestodes in vitro. Experimental Parasitology 48, 164–74.CrossRefGoogle ScholarPubMed
Smyth, J. D. & McManus, D. P. (1989). The Physiology and Biochemistry of Cestodes. Cambridge: Cambridge University Press.CrossRefGoogle Scholar
Studier, F. W. (1973). Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79, 237–48.CrossRefGoogle ScholarPubMed
Tarleton, R. L. & Kemp, W. M. (1981). Demonstration of IgG-Fc and C3 receptors on adult Schistosoma mansoni. Journal of Immunology 126, 379–84.CrossRefGoogle ScholarPubMed
Torpier, G., Capron, A. & Ouaissi, M. A. (1979). Receptor for IgG (Fc) and human beta 2-microglobulin on S. mansoni schistosomula. Nature, London 278, 447–9.CrossRefGoogle ScholarPubMed
Towbin, H., Staehlin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Trimble, J. J. & Lumsden, R. D. (1975). Cytochemical characterization of tegument membrane-associated carbohydrate in Taenia crassiceps larvae. Journal of Parasitology 61, 665–76.Google ScholarPubMed
Willms, K. & Arcos, L. (1977). Taenia solium: host serum proteins on the cysticercus surface identified by an ultrastructural immunoenzyme technique. Experimental Parasitology 43, 396–406.CrossRefGoogle ScholarPubMed