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Suppression of mitogen-induced IL-2Rα expression in PBL by serum from microfilaraemic rats chronically infected with Brugia pahangi

Published online by Cambridge University Press:  01 January 1997

M. SATOH ,
S. TSUKIDATE ,
Y. EISHI ,
N. TAMURA ,
K. YAMAMOTO  and
K. FUJITA
M. SATOH
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan Department of Medical Zoology, Saitama Medical School Moroyama, Iruma-gun, Saitama, 350–04, Japan
S. TSUKIDATE
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
Y. EISHI
Affiliation:
Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
N. TAMURA
Affiliation:
Department of Veterinary Parasitology, Nippon Veterinary and Animal Science University, 1–7-1, Kyounan, Musashino, Tokyo, 180, Japan
K. YAMAMOTO
Affiliation:
Department of Virology, Medical Research Institute, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
K. FUJITA
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
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Abstract

To study immunological responses in filarial infections, interleukin-2 receptor α (IL-2Rα) expression in the peripheral blood lymphocytes (PBL) of Lewis rats infected with Brugia pahangi was observed by flow cytometry. During infection, no increase in IL-2Rα was observed in either microfilaraemic or amicrofilaraemic rats. The PBL of rats were stimulated with phytohaemagglutinin (PHA) and the frequency of IL-2Rα-positivity was examined. After microfilariae appeared, the increase in the rate of IL-2Rα-positivity in microfilaraemic rats was less than in amicrofilaraemic rats. The anergy of IL-2Rα expression in microfilaraemic rats was restored when their lymphocytes were cultured with normal rat serum. IL-2Rα expression in normal uninfected rats decreased when cultured with serum from microfilaraemic rats. These results suggest that microfilariae-related factors in rat serum suppress PHA-induced IL-2Rα expression in PBL and thus inhibit cell proliferation and host immunity.

Keywords

microfilaraemiaserum factorsIL-2Rαimmunosuppression
Type
Research Article
Information
Parasitology , Volume 114 , Issue 1 , January 1997 , pp. 65 - 69
Copyright
© 1997 Cambridge University Press

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