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Substitution of benzimidazole-resistant nematodes for susceptible nematodes in grazing lambs

Published online by Cambridge University Press:  13 November 2006

M.-N. MOUSSAVOU-BOUSSOUGOU ,
A. SILVESTRE ,
J. CORTET ,
C. SAUVE  and
J. CABARET
M.-N. MOUSSAVOU-BOUSSOUGOU
Affiliation:
INRA, UR1282 Animal Infectious Diseases and Public Health, F-37380 Nouzilly, France
A. SILVESTRE
Affiliation:
INRA, UR1282 Animal Infectious Diseases and Public Health, F-37380 Nouzilly, France
J. CORTET
Affiliation:
INRA, UR1282 Animal Infectious Diseases and Public Health, F-37380 Nouzilly, France
C. SAUVE
Affiliation:
INRA, UR1282 Animal Infectious Diseases and Public Health, F-37380 Nouzilly, France
J. CABARET
Affiliation:
INRA, UR1282 Animal Infectious Diseases and Public Health, F-37380 Nouzilly, France
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Abstract

Multi-drug-resistant gastrointestinal nematode parasite populations are becoming more and more prevalent. Since anthelmintic treatments are of limited effectiveness, one solution could be to replace the anthelmintic-resistant population by a susceptible population, in order to re-establish the possibility of drug-based anthelmintic control. We investigated this substitution strategy in 4 paddocks of 0·7 ha, each of which was seeded with a benzimidazole-resistant Teladorsagia circumcincta population. The proportion of benzimidazole-resistant worms in these paddocks ranged from 20% to 89%. A 2-step replacement was performed: first, the paddocks were not grazed for 6 months (from December to July), and then the grass was cut to eliminate any residual infective larvae, before contaminating each of the paddocks with 10 seeder lambs experimentally infected with a benzimidazole-susceptible strain of T. circumcincta (from July to November). At the end of the experiment, all the populations on the 4 paddocks were phenotypically benzimidazole-susceptible, but genotyping indicated that 2 populations harboured 1% and 3% resistant worms respectively. This study demonstrates that nematode replacement is feasible in temperate areas, using semi-intensive stock management, even when the initial levels of benzimidazole-resistance are very high. Further research should next assess replacing the whole community to cope with the species diversity observed under field conditions.

Keywords

nematode controlanthelmintic resistancereplacementsynthetic strainbenzimidazoleTeladorsagia circumcincta
Type
Research Article
Information
Parasitology , Volume 134 , Issue 4 , April 2007 , pp. 553 - 560
Copyright
© 2006 Cambridge University Press

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