Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-05T04:12:56.913Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies of the amino acids of Rhodnius prolixus. II. Analysis of the excretory material

Published online by Cambridge University Press:  06 April 2009

J. S. Harington
J. S. Harington
Affiliation:
Department of Entomology, London School of Hygiene and Tropical Medicine*
Get access Rights & Permissions [Opens in a new window]

Extract

1. A qualitative paper chromatographic analysis of the free amino acids in Rhodnius urine and excreta was made: eleven amino acids or related compounds were detected, cystine and cysteic acid in urine, and histidine, histamine, leucine, taurine, glycine, valine, phenylalanine and alanine in the excretory fractions.

2. In addition to these, three yellow pigment spots were separated from the excreta; one of these is of unknown composition and two are peptides.

3. A possible relationship between certain of the excretory amino acids and haemolymph ones is discussed.

I am greatly indebted to the late Professor P. A. Buxton, C.M.G., F.R.S., for his stimulating criticism during this study, and to the members of the School for very useful discussion, and to Mr Paul Baily for performing the column chromatographic analyses on my behalf. The study forms part of a Thesis for the degree of Ph.D. approved by the University of London, and was financed by the South African Council for Scientific and Industrial Research. I wish to acknowledge their generous financial support.

Type
Research Article
Information
Parasitology , Volume 51 , Issue 3-4 , November 1961 , pp. 319 - 326
Copyright
Copyright © Cambridge University Press 1961

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Auclair, J. L. & Dubreuil, R. (1952). A simple ultramicromethod for the quantitative estimation of amino acids by paper partition chromatography. Canad. J. Zool. 30, 109.CrossRefGoogle Scholar
Awapara, J. (1948). Application of paper chromatography to the estimation of the free amino acids in tissues. Arch. Biochem. 19, 172.Google Scholar
Beadle, L. C. & Shaw, J. (1950). The retention of salt and the regulation of the non-protein nitrogen fraction in the blood of the aquatic larva, Sialis lutaria. J. Exp. Biol. 27, 96.CrossRefGoogle Scholar
Brown, A. W. A. (1937). Studies on the excreta of a grasshopper (Melanoplus bivittatus Say.). J. Exp. Biol. 14, 87.CrossRefGoogle Scholar
Craig, R. (1960). The physiology of excretion in the insect. Ann. Rev. Ent. 5, 53.Google Scholar
Dent, C. E. (1947). The amino-aciduria in Fanconi syndrome. Biochem. J. 41, 240.CrossRefGoogle ScholarPubMed
Dent, C. E. (1948). A study of the behaviour of some sixty amino-acids and other ninhydrin-reacting substances on phenol-‘collidine’ filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids. Biochem. J. 43, 169.Google Scholar
Fukuda, T. (1950). Discrimination by paper chromatography of the free amino acids in the blood of the silkworm. J. seric. Sci., Tokyo, 19, 372.Google Scholar
Fukuda, T. (1956). Conversion of phenylalanine into tyrosine in the silkworm larva (Bombyxmori). Nature, Lond., 177, 429.CrossRefGoogle Scholar
Fukuda, T. & Sakano, S. (1950). Discrimination by paper chromatography of the free amino acids in the blood of silkworm pupae. J. seric. Sci., Tokyo, 19, 490.Google Scholar
Good, P. M. & Johnson, A. W. (1949). Paper chromatography of pterins. Nature, Lond., 163, 31.Google Scholar
Harington, J. S. (1956). Histamine and histidine in excreta of the blood-sucking bug, Rhodnius prolixus. Nature, Lond., 178, 268.Google Scholar
Harington, J. S. (1961 a). Studies of the amino acids of Rhodnius prolixus. I. Analysis of the haemolymph. Parasitology, 51, 309–318.Google Scholar
Harington, J. S. (1961 b). Changes in the free amino acid concentration in Rhodnius prolixus haemolymph from feeding to ecdysis. J. Ent. Soc. S. Afr., 24, 216, 218.Google Scholar
Hopkins, F. G. (1896). The pigments of the Pteridae: a contribution to the study of excretory substances which function in ornament. Phil. Trans. 186, 661.Google Scholar
Kawase, (1915). In Japanese; seen in Yoshitake & Aruga (1950 a).Google Scholar
Moore, S., Spackman, D. H. & Stein, W. H. (1958). An improved system for the chromatography of amino acids on sulfonated polystyrene resins. Anal. Chem. 30, 1185.Google Scholar
Partridge, S. M. (1948). Filter-paper partition chromatography of sugars. 1. General description and application to the qualitative analysis of sugars in apple juice, egg white, and foetal blood of sheep. Biochem. J. 42, 238.Google Scholar
Patton, W. S. (1953). In Insect Physiology, p. 397. Editor: Roeder., K. D.London: Chapman and Hall.Google Scholar
Wigglesworth, V. B. (1931). The physiology of excretion in a blood-sucking insect, Rhodnius prolixus (Hemiptera, Reduviidae). 1. Composition of the Urine. J. Exp. Biol. 8, 411.Google Scholar
Yoshitake, N. & Aruga, H. (1950 a). Studies on the amino acids in the silkworm. IV. On the free amino acids in the silkworm faeces. J. seric. Sci., Tokyo, 19, 536.Google Scholar
Yoshitake, N. & Aruga, H. (1950 b). Studies on the amino acids in the silkworm. V. On the free amino acids in the pupa and moth. J. seric. Sci., Tokyo, 19, 538.Google Scholar