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Specific sites in the Beta Interaction Domain of a schistosome Ca2+ channel β subunit are key to its role in sensitivity to the anti-schistosomal drug praziquantel

Published online by Cambridge University Press:  17 October 2003

A. B. KOHN ,
J. M. ROBERTS-MISTERLY ,
P. A. V. ANDERSON ,
N. KHAN  and
R. M. GREENBERG
A. B. KOHN
Affiliation:
Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA
J. M. ROBERTS-MISTERLY
Affiliation:
Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA
P. A. V. ANDERSON
Affiliation:
Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA Department of Neuroscience, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA Department of Physiology and Functional Genomics, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA
N. KHAN
Affiliation:
Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA
R. M. GREENBERG
Affiliation:
Whitney Laboratory, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA Department of Neuroscience, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA Department of Pathobiology, University of Florida, 9505 Ocean Shore Boulevard, St Augustine, FL 32080, USA
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Abstract

Praziquantel, the drug of choice against schistosomiasis, disrupts calcium (Ca2+) homeostasis in schistosomes via an unknown mechanism. Voltage-gated Ca2+ channels are heteromultimeric transmembrane protein complexes that contribute to impulse propagation and also regulate intracellular Ca2+ levels. β subunits modulate the properties of the pore-forming α1 subunit of high voltage-activated Ca2+ channels. Unlike other Ca2+ channel β subunits, which have current stimulatory effects, a β subunit subtype found in S. mansoni (SmβA) and S. japonicum (Sjβ) dramatically reduces current levels when co-expressed with Ca2+ channel α1 subunits in Xenopus oocytes. It also confers praziquantel sensitivity to the mammalian Cav2.3 α1 subunit. The Beta Interaction Domains (BIDs) of SmβA and Sjβ lack 2 conserved serines that each constitute a consensus site for protein kinase C (PKC) phosphorylation. Here, we use site-directed mutagenesis of schistosome β subunits to show that these unique functional properties are correlated with the absence of these consensus PKC sites in the BID. Furthermore, a second schistosome β subunit subtype contains both serines in the BID, enhances currents through α1 subunits, and does not confer praziquantel sensitivity. Thus, phosphorylation sites in the BID may play important roles in defining the modulatory properties and pharmacological sensitivities of schistosome Ca2+ channel β subunits.

Keywords

praziquantelCa2+ channelschistosomiasisprotein kinase C
Type
Research Article
Information
Parasitology , Volume 127 , Issue 4 , October 2003 , pp. 349 - 356
Copyright
2003 Cambridge University Press

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