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A small ciliary surface glycoprotein of the monogenean parasite Neobenedenia girellae acts as an agglutination/immobilization antigen and induces an immune response in the Japanese flounder Paralichthys olivaceus

Published online by Cambridge University Press:  11 July 2005

A. HATANAKA ,
N. UMEDA ,
S. YAMASHITA  and
N. HIRAZAWA
A. HATANAKA
Affiliation:
Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan
N. UMEDA
Affiliation:
Marine Biological Technology Center of Nippon Suisan Kaisha Ltd, 508-8 Ariakeura Tsurumi-Cho, Minamiamabegun, Oita 876-1204, Japan
S. YAMASHITA
Affiliation:
Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan
N. HIRAZAWA
Affiliation:
Central Research Laboratories of Nippon Suisan Kaisha Ltd, 559-6 Kitano-Machi, Hachioji, Tokyo 192-0906, Japan
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Abstract

The capsalid monogenean Neobenedenia girellae, a parasite of seawater fishes, was found to express an antigen that elicits antibodies in rabbits, and these antibodies had agglutination/immobilization activity against N. girellae larvae (oncomiracidia) in vitro. Indirect immunofluorescence staining of N. girellae oncomiracidia showed that this agglutination/immobilization antigen was expressed on the surface of cilia. An intraperitoneal injection of ciliary proteins (either sonicated or intact) with adjuvant also elicited agglutinizing/immobilizing antibodies in sera from Japanese flounder, Paralichthys olivaceus. These antisera showed a clear correlation between anti-ciliary antibody levels (measured by enzyme-linked immunosorbent assays) and their agglutination/immobilization activity. Anti-ciliary antibody levels in Japanese flounder reached a plateau at 39 days after booster immunization and were significantly higher in the two immunized groups (injection of sonicated or intact cilia) as compared with control fish (injection of bovine serum albumin; ANOVA, Tukey's test, P<0·01). Anti-ciliary antibodies were also found in fish mucus; however, there was no correlation between fish serum anti-ciliary antibody levels and mucus antibody levels. A Triton X-114-soluble 8 kDa glycoprotein of the ciliary integral membrane fraction is a plausible candidate for the agglutination/immobilization antigen based on SDS-polyacrylamide gel electrophoresis and immunoblot analyses with rabbit and fish antisera.

Keywords

parasitemonogeneanNeobenedenia girellaeciliary proteinimmunoresponseagglutination/immobilization antigenParalichthys olivaceus
Type
Research Article
Information
Parasitology , Volume 131 , Issue 5 , November 2005 , pp. 591 - 600
Copyright
© 2005 Cambridge University Press

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