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Short-term culture of Plasmodium knowlesi

Published online by Cambridge University Press:  06 April 2009

G. A. Butcher  and
S. Cohen
G. A. Butcher
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, S.E. 1
S. Cohen
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, S.E. 1
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Extract

The culture method developed by Geiman and co-workers has been modified to give average multiplication rates for Plasmodium knowlesi of at least six-fold in 24 h.

Individual modifications have been assessed on the basis of parasite multiplication and [3H]leucine incorporation into parasite protein. The second cycle of in vitro development which is particularly influenced by the conditions of culture was improved by: (i) culture of washed parasitized red cells in a medium containing dialysed homologous serum screened for its ability to support parasite growth; (ii) addition of ATP, Co-enzyme A (or pantothenate) and glutamine.

We thank Mr E. D. Dennis and Miss Susan Wanstall for technical assistance. This work was supported by grants from the Medical Research Council and the World Health Organization.

Type
Research Article
Information
Parasitology , Volume 62 , Issue 2 , April 1971 , pp. 309 - 320
Copyright
Copyright © Cambridge University Press 1971

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References

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