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The serotoninergic, cholinergic and peptidergic components of the nervous system in the monogenean parasite,Diclidophora merlangi: a cytochemical study

Published online by Cambridge University Press:  06 April 2009

A. G. Maule ,
D. W. Halton ,
C. F. Johnston ,
C. Shaw  and
I. Fairweather
A. G. Maule
Affiliation:
School of Biology and Biochemistry, The Queen's university of Belfast, Belfast BT7 1NN, U.K.
D. W. Halton
Affiliation:
School of Biology and Biochemistry, The Queen's university of Belfast, Belfast BT7 1NN, U.K.
C. F. Johnston
Affiliation:
Department of Medicine, The Queen's University of Belfast, Belfast BT7 INN, U.K.
C. Shaw
Affiliation:
Department of Medicine, The Queen's University of Belfast, Belfast BT7 INN, U.K.
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's university of Belfast, Belfast BT7 1NN, U.K.
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Summary

Confocal scanning laser microscopy has been employed with immunocytochemical techniques to map the distribution of serotoninergic and peptidergic components in the nervous system of the monogenean gill-parasite, Diclidophora merlangi; results are compared with the distribution of cholinergic components, following histochemical staining for cholinesterase activity. While all three neurochemical elements are present in the central and peripheral nervous systems, the cholinergic and peptidergic systems dominate the CNS, whereas the PNS has a majority of serotoninergic nerve fibres. The cholinergic and peptidergic neuronal pathways overlap extensively in staining patterns, suggesting possible co-localization of acetylcholine and neuropeptides. Within the peptidergic nervous system, immunoreactivity to the pancreatic polypeptide family of peptides and FMRFamide were the most prevalent.

Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 255 - 273
Copyright
Copyright © Cambridge University Press 1990

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