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Schistosoma mansoni: the structure and elemental composition of pre-acetabular penetration gland cell secretion in pre-emergent cercariae

Published online by Cambridge University Press:  06 April 2009

T. W. Davies
T. W. Davies*
Affiliation:
Department of Zoology, University college, Cardiff, Wales
*
*Deceased. All correspondence regarding this paper should be sent to Professor D. A. Erasmus at the above address.
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Summary

The structure and elemental composition of the secretory products of the pre-acetabular penetration gland cells of pre-emergent cercariae of Schistosoma mansoni have been investigated at an ultrastructural level using X-ray probe microanalysis. The secretion contains 2 types of bodies, one homogeneous and one heterogeneous with electron-lucent areas. Using a variety of fixation and sectioning techniques it has been shown that the apparent electron density of the homogeneous bodies (Type B) is the result of interaction with osmium tetroxide and stain. The small electron-lucent areas of the other type of secretory body (Type A) represent regions where granules have been leached out by processing media. X-ray analysis of cercariae still within sporocysts has indicated that these granules are very rich in calcium, and it is suggested that the calcium is absorbed by the developing cercariae through the sporocyst wall from the molluscan haemolymph.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 1 , August 1983 , pp. 55 - 60
Copyright
Copyright © Cambridge University Press 1983

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References

REFERENCES

Cheng, T. C. & Bier, J. W. (1972). Studies on molluscan schistosomiasis. An analysis of the development of the cercaria of Schistosoma mansoni. Parasitology 64, 129–41.CrossRefGoogle ScholarPubMed
Dorsey, C. H. (1975) Schistosoma mansoni: Development of acetabular glands of cercaria at ultrastructural level. Experimental Parasitology 37, 37–50.CrossRefGoogle ScholarPubMed
Dorsey, C. H. & Stirewalt, M. A. (1971). Schistosoma mansoni: Fine structure of cercarial acetabular glands. Experimental Parasitology 30, 199–214.CrossRefGoogle ScholarPubMed
Dorsey, C. H. & Stirewalt, M. A. (1977). Schistosoma mansoni: Localisation of calcium-detecting reagents in electron-lucent areas of specific preacetabular gland granules. Zeitschrift für Parasitenkunde 54, 165–73.CrossRefGoogle ScholarPubMed
Dresden, M. H. & Asch, H. L. (1972). Proteolytic enzymes in extracts of Schistosoma mansoni cercariae. Biochimica et biophysica acta 289, 378–84.CrossRefGoogle ScholarPubMed
Dresden, M. H. & Asch, H. L. (1977) Calcium carbonate content of the pre-acetabular glands of Schistosoma mansoni cercariae. Journal of Parasitology 63, 163–5.CrossRefGoogle Scholar
Dresden, M. H. & Edlin, E. M. (1975). Schistosoma mansoni: calcium content of cercariae and its effects on protease activity in vitro. Journal of Parasitology 61, 398–402.CrossRefGoogle Scholar
Ebrahimzadeh, A. & Kraft, M. (1971). Ultrastrukturelle Untersuchungen zur Anatomie Cercarien von Schistosoma mansoni. III. Das Drusensystem. Zeitschrift für Parasitenkunde 36, 291–303.CrossRefGoogle Scholar
Erasmus, D. A. (1967) Ultrastructural observations on the reserve bladder system of Cyathocotyle bushiensis Khan, 1962, with special reference to lipid excretion. Journal of Parasitology 53, 525–36.CrossRefGoogle ScholarPubMed
Erasmus, D. A. & Davies, T. W. (1979). Schistosoma mansoni and S. haematobium: calcium metabolism of the vitelline cell. Experimental Parasitology 47, 91–106.CrossRefGoogle ScholarPubMed
Erasmus, D. A., Popiel, I. & Shaw, J. R. (1982). A comparative study of the vitelline cell in Schistosoma mansoni, S. haematobium, S.japonicum and S. mattheii. Parasitology 84, 283–7.CrossRefGoogle Scholar
Morgan, A. J., Davies, T. W. & Erasmus, D. A. (1975). Changes in the concentration and distribution of elements during electron microscope preparative procedures. Micron 6, 11–23.Google Scholar
Pax, R., Bennett, J. L. & Fetterer, R. (1978). A benzodiazepine derivative and praziquantel: effects on musculature of Schistosoma mansoni and S. japonicum. Naunyn-Schmeideberg's Archives of Pharmacology 304, 309–15.CrossRefGoogle Scholar
Shaw, M. K. & Erasmus, D. A. (1982). Schistosoma mansoni: the presence and ultrastructure of vitelline cells in adult males. Journal of Helminthology 56, 51–3.CrossRefGoogle ScholarPubMed
Shaw, M. K., Davies, T. W. & Erasmus, D. A. (1982). The demonstration of calcium in the axonal granules of the peripheral nervous system of male Schistosoma mansoni by X-ray microanalysis. Cell and Tissue Research 222, 597–604.CrossRefGoogle ScholarPubMed
Simkiss, K. (1976). Intracellular and extracellular routes in biomineralization. In Calcium in Biological Systems. Symposia for the Society for Experimental Biology, vol. 30, (ed. Duncan, C. J.), pp. 423–446. London and New York: Cambridge University Press.Google Scholar
Stirewalt, M. A. & Kruidenier, F. J. (1961). Activity of the acetabular secretory apparatus of cercariae of Schistosoma mansoni under experimental conditions. Experimental Parasitology 11, 191–211.CrossRefGoogle ScholarPubMed