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Schistosoma mansoni: in vitro conversion of cercariae to schistosomula

Published online by Cambridge University Press:  06 April 2009

Extract

Schistosoma mansoni cercariae were incubated for 3–5 h at 37°C in various test solutions, and the rate and extent of conversion of th ecercariae to schistosomula were determined. Criteria used to identify schistosomula included: (1) loss of cercariai tail, (2) viability of organisms in saline but not in water, (3) pre-acetabular gland evacuation and (4) ability to survive in culture.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

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References

Austin, F., Frappaola, P., Gilbert, B., Landis, W., da Rosa, M. N. & Stirewalt, M. (1974). Further studies of Schistosoma mansoni cercarial stimulation by crude egg lecithin and other lipids. Parasitology 64, 455–63.CrossRefGoogle Scholar
Clegg, J. A. (1965). In vitro cultivation of Schistosoma mansoni. Experimental Parasitology 16, 133–47.CrossRefGoogle ScholarPubMed
Clegg, J. A. & Smithers, S. R. (1972). The effect of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal of Parasitology 2, 7998.CrossRefGoogle ScholarPubMed
Colley, D. G. & Wikel, S. K. (1974). Schistosoma mansoni: Simplified method for the production of schistosomules. Experimental Parasitology 35, 261–4.CrossRefGoogle ScholarPubMed
Eveland, L. K. (1972). Schistosoma mansoni: Conversion of cercariae to schistosomula. Experimental Parasitology 32, 261–4.CrossRefGoogle ScholarPubMed
Gazzinelli, G., de Oliveira, C. C., Figueiredo, E. A., Pereira, L. H., Coelho, P. M. Z. & Pellegrino, J. (1973). Schistosoma mansoni: Biochemical evidence for morphogenetic change from cercaria to schistosomule. Experimental Parasitology 34, 181–8.CrossRefGoogle ScholarPubMed
Gilbert, B., da Rosa, M., Borojevic, R. & Pellegrino, J. (1972). Schistosoma mansoni: in vitro transformation of cercariae into schistosomula. Parasitology 64, 333–9.CrossRefGoogle ScholarPubMed
Hirsch, J. G. & Fedorko, M. E. (1968). Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and ‘postfixation’ in uranyl acetate. Journal of Cell Biology 38, 615–27.CrossRefGoogle ScholarPubMed
Lewert, R. M. & Para, J. B. (1966). The physiological incorporation of carbon-14 in Schistosoma mansoni cercariae. Journal of Infectious Diseases 116, 171–82.CrossRefGoogle ScholarPubMed
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: Defined system for stepwise transformation of cercariae to schistosomula in vitro. Experimental Parasitology 36, 360–72.CrossRefGoogle Scholar
Stirewalt, M. A. (1966). Definition and collection in quantity of schistosomules of Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 352–60.CrossRefGoogle ScholarPubMed
Stirewalt, M. A. & Uy, A. (1969). Schistosoma mansoni: Cercarial penetration and schistosomule collection in an in vitro system. Experimental Parasitology 26, 1728.CrossRefGoogle Scholar
Tiba, Y., Holanda, J. C., Ramalho-Pinto, F. J., Gazzinelli, G. & Pellegrino, J. (1974). Schistosomula (Schistosoma mansoni) obtained in vitro: viability in culture and infectivity for mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 68, 72.CrossRefGoogle ScholarPubMed