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Schistosoma mansoni: evidence for a role of serum factors in protecting artificially transformed schistosomula against antibody-mediated killing in vitro

Published online by Cambridge University Press:  06 April 2009

C. A. P. Tavares ,
Rita C. Soares ,
P. M. Z. Coelho  and
G. Gazzinelli
C. A. P. Tavares
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
Rita C. Soares
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
P. M. Z. Coelho
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
G. Gazzinelli
Affiliation:
Departamento de Bioquímica-Immunologia, Instituto de Ciäcias Biológicas da UFMG, C.P. 2486, 3.000 Belo Horizonte, Brasil
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Summary

Artificially transformed schistosomula of Schistosoma mansoni develop a consistent but small protection against the lethal effects of antibody plus complement when cultured for 24 h in a chemically defined medium. In contrast, they become rapidly resistant to antibody plus complement, when cultured in the presence of a complex medium consisting of equal parts of heat-inactivated rabbit serum and Earle's/lactalbumin or in defined medium supplemented with small amounts of heat-inactivated rabbit serum. Sephadex G-200 gel filtration revealed that the protective factor in rabbit serum is a macromolecule with a molecular weight between 7 and 19S. Parasites cultured at 10 °C or in the presence of 200 μg of puromycin show less serum-induced protection against the lethal effects of antibody plus complement than do controls.

Type
Research Article
Information
Parasitology , Volume 77 , Issue 2 , October 1978 , pp. 225 - 233
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Clegg, J. A. (1974). Host antigens and the immune response in schistosomiasis. Parasites in the immunized host: mechanisms of survival. Ciba Foundation Symposium, 25 (now series), 161–83.CrossRefGoogle Scholar
Clegg, J. A. & Smithers, S. R. (1972). The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 79–88.CrossRefGoogle ScholarPubMed
Dean, D. A. (1977). Decreased binding of cytotoxic antibody by developing Shistosoma mansoni. Evidence for a surface change independent of host antigen absorption and membrane turnover. Journal of Parasitology 63, 418–26.CrossRefGoogle Scholar
Gazzinelli, G., Oliveira, C. C., Figueiredo, E. A., Pereira, L. H., Coelho, P. M. Z. & Pellegrino, J. (1973). Schistosoma mansoni: biochemical evidence for morphogenetic change of cercaria to schistosomule. Experimental Parasitology 34, 181–8.CrossRefGoogle ScholarPubMed
Goldring, O. L., Clegg, J. A., Smithers, S. R. & Terry, R. J. (1976). Acquisition of human blood group antigens by Schistosoma mansoni. Clinical and Experimental Immunology 26, 181–7.Google ScholarPubMed
Goldring, O. L., Sher, A., Smithers, S. R. & McLaren, D. J. (1977). Host antigens and parasite antigens of murine Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 71, 144–8.CrossRefGoogle ScholarPubMed
Hockley, D. J. & Mclaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worms. International Journal for Parasitology 3, 13–25.CrossRefGoogle Scholar
McLaren, D. J., Clegg, J. A. & Smithers, S. R. (1975). Acquisition of host antigens by young Schistosoma mansoni in mice: correlation with failure to bind antibody in vitro. Parasitology 70, 67–75.CrossRefGoogle ScholarPubMed
McLaren, D. J., Hockley, D. J., Goldring, O. L. & Hammond, B. J. (1978). A Freese-fracture study of the developing tegumental outer membrane of Shistosoma mansoni. Parasitology 76, 327–348.CrossRefGoogle Scholar
Nagai, Y., Gazzinelli, G., Moraes, G. W. G. & Pellegrino, J. (1977). Protein synthesis during cercaria–schistosomulum transformation and early development of the Schistosoma mansoni larvae. Comparative Biochemistry and Physiology 57B, 27–30.Google ScholarPubMed
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: defined system for stepwise transformation of cercariae to schistosomule in vitro. Experimental Parasitology 36, 360–73.CrossRefGoogle ScholarPubMed
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E. & Pellegrino, J. (1975). Factors affecting surface changes in intact cercariae and cercarial bodies of Schistosoma mansoni. Parasitology 71, 19–25.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1976). The immunology of schistosomiasis. In Advances in Parasitology, vol. 7 (ed. B. Dawes), pp. 41–93. London and New York: Academic Press.Google Scholar
Smithers, S. R., Terry, R. J. & Hockley, D. J. (1969). Host antigens in schistosomiasis. Proceedings of the Royal Society of London, B 171, 483–94.Google ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1977). The formation and turnover of the membranocalix on the tegument of Schistosoma mansoni. Parasitology 74, 61–71.CrossRefGoogle ScholarPubMed