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The role of acidic organelles in the development of schistosomula of Schistosoma mansoni and their response to signalling molecules

Published online by Cambridge University Press:  01 November 2004

B. H. AL-ADHAMI ,
C. NOBLE ,
O. SHARAF ,
J. THORNHILL ,
M. J. DOENHOFF  and
J. R. KUSEL
B. H. AL-ADHAMI
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
C. NOBLE
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
O. SHARAF
Affiliation:
Division of Infection and Immunity, The Joseph Black Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
J. THORNHILL
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
M. J. DOENHOFF
Affiliation:
School of Biological Sciences, Bangor University of Wales, Bangor, North Wales LL57 2UW, UK
J. R. KUSEL
Affiliation:
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Science, University of Glasgow, Glasgow G12 8QQ, UK
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Abstract

The cercariae of Schistosoma mansoni become transformed into schistosomula during host skin penetration. We have found that large acidophilic compartments are detected in schistosomula but not in cercariae or in any other stages of the parasite by use of the fluorescent dye LysoTracker, a dye specific for mammalian lysosomes. Some of these large acidic compartments incorporated monodansylcadaverine, a specific dye for autophagosomes. We have used potent inhibitors (wortmannin and 3-methyladenine) and a potent inducer (starvation) of autophagy to show that the pathway to the formation of the acidic compartments requires specific molecular signals from the environment and from the genome. Certain doses of ultraviolet light inhibited significantly the formation of the acidic compartments, which may indicate disruption of the lysosome/autophagosome pathway. We have also defined two proteins that are commonly associated with lysosomes and autophagosomes in mammalian cells, the microtubule-associated membrane protein (MAP-LC3) and lysosome-associated membrane protein (LAMP-1), in extracts of schistosomula. We suggest that the autophagy pathway could be developed in transformed schistosomula.

Keywords

schistosomeslysosomesautophagosomesLysoTracker dyemonodansylcadaverine
Type
Research Article
Information
Parasitology , Volume 130 , Issue 3 , March 2005 , pp. 309 - 322
Copyright
2005 Cambridge University Press

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