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Rickettsia-like organisms and chitinase production in relation to transmission of trypanosomes by tsetse flies

Published online by Cambridge University Press:  06 April 2009

S. C. Welburn ,
K. Arnold ,
I. Maudlin  and
G. W. Gooday
S. C. Welburn
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Langford House, Langford, Bristol BS18 7DU
K. Arnold
Affiliation:
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Aberdeen AB9 1AS
I. Maudlin
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Langford House, Langford, Bristol BS18 7DU
G. W. Gooday
Affiliation:
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Aberdeen AB9 1AS
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Summary

Rickettsia-like organisms (RLO) from tsetse midguts and mosquito cell cultures showed high levels of endochitinase activity. A line of Glossina morsitans morsitans highly susceptible to midgut trypanosome infection and with high incidence of RLO infection showed significantly greater chitinolytic activity than G. austeni which had low RLO incidence and were correspondingly refractory to midgut infection. Midgut infection rates of Trypanosoma brucei rhodesiense in G. m. morsitans showed a dose-related increase when flies were fed N-acetyl-D-glucosamine (GlcNAc) in the infective meal and for 4 subsequent days. A model is proposed for susceptibility to trypanosome infection based on the generation of GlcNAc by RLO endochitinase activity in tsetse pupae inhibiting midgut lectin in teneral flies.

Keywords

chitinasetrypanosomiasistsetserickettsia-like organismslectins
Type
Research Article
Information
Parasitology , Volume 107 , Issue 2 , August 1993 , pp. 141 - 145
Copyright
Copyright © Cambridge University Press 1993

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