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Reservosome: an endocytic compartment in epimastigote forms of the protozoan Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae). Correlation between endocytosis of nutrients and cell differentiation

Published online by Cambridge University Press:  14 September 2004

R. C. B. Q. FIGUEIREDO ,
D. S. ROSA ,
Y. M. GOMES ,
M. NAKASAWA  and
M. J. SOARES
R. C. B. Q. FIGUEIREDO
Affiliation:
Departamento de Biologia Celular Ultrastructura, Centro de Pesquisas Aggeu Megalhães/FIOCRUZ, Av. Moraes Rego s/n°, 50670-420 Recife, PE, Brazil
D. S. ROSA
Affiliation:
Universidade Federal de São Paulo, Departamento de Microbiologia Imunobiologia e Parasitologia – UNIFESP, Rua Botucatu 862/6° andar, Vila Clementino, 04023-062 São Paulo, SP, Brazil
Y. M. GOMES
Affiliation:
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Av. Moraes Rego s/n°, 50670-420 Recife, PE, Brazil
M. NAKASAWA
Affiliation:
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Av. Moraes Rego s/n°, 50670-420 Recife, PE, Brazil
M. J. SOARES
Affiliation:
Departamento de Ultra-estrutura e Biologia Celular, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil
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Abstract

Reservosomes are large membrane-bound organelles found at the posterior end of epimastigote forms of Trypanosoma cruzi, but absent in amastigotes and trypomastigotes. We have transferred bloodstream trypomastigotes to LIT medium supplemented with gold-labelled transferrin in order to analyse, at the ultrastructural level, the occurrence of reservosomes and endocytosis during the trypomastigote to epimastigote differentiation. After 24 h, the trypomastigotes differentiated into amastigotes, which adhered to each other forming large clusters. Electron-dense vesicles were detected close to the Golgi complex in cells with intermediary characteristics between amastigotes and epimastigotes, but typical reservosomes at the posterior cell tip were still absent. Transferrin–gold complexes were observed only bound to the surface of clustered cells. After 72 h, epimastigotes were observed being released from the clusters and free-swimming epimastigotes appeared, containing electron-dense vesicles at their posterior region. Typical reservosomes, labelled with transferrin–gold, were observed only in free-swimming epimastigotes. When fully differentiated epimastigotes were incubated with transferrin–gold complexes and then processed for the immunocytochemical detection of cysteine proteinase, all reservosomes were positive for the enzyme, but co-localization of both markers did not occur in all organelles. Our data demonstrate that in T. cruzi epimastigotes endocytosis is strongly related to reservosome biogenesis during the trypomastigote to epimastigote differentiation process.

Keywords

endocytosisKinetoplastidareservosomesTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 129 , Issue 4 , October 2004 , pp. 431 - 438
Copyright
2004 Cambridge University Press

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