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Researches on the intestinal protozoa of monkeys and man

IX. The life-history of Entamoeba coli, with special reference to metacystic development

Published online by Cambridge University Press:  06 April 2009

Clifford Dobell
Clifford Dobell
Affiliation:
National Institute for Medical Research, London, N.W. 3
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1. The life-history of Entamoeba coli (from a marmoset, several macaques, and Man), as observable in cultures, is here described and illustrated. It is found to be similar, in general outline, to that of E. histolytica, but to differ considerably in its excystation and metacystic stages.

2. At excystation, the entire contents of the cyst usually escapes, as a single multinucleate amoeba, through a large opening in the cyst wall, which is composed of two layers.

3. Normal ripe cysts frequently lose 1 to 4 of their 8 original nuclei before hatching, thus becoming “infranucleate” (i.e. containing only 4 to 7 nuclei instead of the “typical” 8).

4. During the metacystic stages no nuclear divisions occur. The hatched amoeba eventually produces, by a series of binary cytoplasmic fissions with random nuclear distribution, as many uninucleate amoebulae as it originally contained nuclei.

5. An originally 8-nucleate cyst may therefore produce 8 amoebulae finally –never more: but owing to the frequent development of infranucleate forms, it often produces only 7, 6, 5, or 4.

6. Development is complicated by occasional division into two within the cyst before hatching, or at the moment of hatching—such divisions being seen in both “typical” and “infranucleate” cysts; and also by the occurrence of supernucleate cysts (containing more than 8 nuclei), which are often viable but usually develop in an infranucleate condition. Consequently, amoebae containing any number of nuclei from 1 to 8—or even more—may hatch from the cysts of this species (unlike those of any other hitherto described).

7. The uninucleate amoebulae resulting from the metacystic divisions are young trophic amoebae—not gametes—and no sexual stages occur in the life-cycle. Division of the trophic amoebae is briefly described and figured.

8. The life-cycle of E. coli is finally compared with that of E. histolytica and related species: the relevant observations of other workers are briefly analysed: and the taxonomic implications are noted.

A memorandum is added on the genus Entamoeba, in an attempt to clarify the nomenclatural and taxonomic confusion created by Opinion 99 promulgated by the International Commission on Zoological Nomenclature. This leads to the conclusion that the Opinion itself is a dead letter, though the genus Entamoeba still lives and should therefore be retained, for the time being, with E. coli Casagrandi & Barbagallo, 1895, as its type.

Type
Research Article
Information
Parasitology , Volume 30 , Issue 2 , June 1938 , pp. 195 - 238
Copyright
Copyright © Cambridge University Press 1938

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