Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-28T02:34:04.364Z Has data issue: false hasContentIssue false
  • English
  • Français

Regulation of macrophage-mediated larvicidal activity in Echinococcus granulosus and Mesocestoides corti (Cestoda) infection in mice

Published online by Cambridge University Press:  06 April 2009

P. Jenkins ,
J. B. Dixon ,
N. K. Rakha  and
S. D. Carter
P. Jenkins
Affiliation:
Departments of Veterinary Pathology
J. B. Dixon
Affiliation:
Departments of Veterinary Pathology
N. K. Rakha
Affiliation:
Departments of Veterinary Pathology
S. D. Carter
Affiliation:
Departments of Veterinary Pathology Veterinary Clinical Science, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
Get access Rights & Permissions [Opens in a new window]

Summary

Killing of metacestodes by normal or post-infection macrophages and the regulation of this activity by cytokines were studied in vitro. The protoscolecidal activity of normal macrophages against Echinococcus granulosus was inhibited by a product of naive T-enriched lymphocytes co-cultured with protoscoleces (PSC). By contrast, supernates from co-cultures of Mesocestoides corti tetrathyridia (MCT) and T-enriched or B-enriched normal lymphocytes increased killing of MCT by normal macrophages. Larvicidal activity (against both PSC and MCT) was enhanced by high concentrations of macrophage-activating factors produced by Con A-stimulated rat lymphocytes (Con A-LK), but was reduced by low concentrations of these factors.

Keywords

Echinococcus granulosusMesocestoides corticestodemacrophagecytokine
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 309 - 315
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ali-Khan, Z. & Siboo, R. (1980). Pathogenesis and host response in subcutaneous alveolar hydatidosis. 1. Histogenesis of alveolar cyst and qualitative analysis of the inflammatory infiltrates. Zeitschrift für Parasitenkunde 62, 241–54.CrossRefGoogle Scholar
Allan, O., Jenkins, P., Connor, R. J. & Dixon, J. B. (1981). A study of immunoregulation of Balb/c mice by Echinococcus granulosus equinus during prolonged infection. Parasite Immunology 3, 137–42.CrossRefGoogle ScholarPubMed
Baron, R. W. & Tanner, C. E. (1977). Echinococcus multilocularis in the mouse: the protoscolecidal activity of peritoneal macrophages. International Journal for Parasitology 7, 489–95.CrossRefGoogle Scholar
Buchmuller, Y. & Mauel, J. (1979). Studies on the mechanisms of macrophage activation. II. Parasite destruction in macrophages activated by supernates from Con A-stimulated lymphocytes. Journal of Experimental Medicine 150, 359–70.CrossRefGoogle Scholar
Connor, R. J. (1980). Experimental infection of mice with Echinococcus granulosus of British origin. Annals of Tropical Medicine and Parasitology 74, 97–9.CrossRefGoogle ScholarPubMed
Cox, D. A., Dixon, J. B. & Marshall-Clarke, S. (1986). Transformation induced by Echinococcus granulosus protoscoleces in unprimed mouse spleen cells: Identity and MHC restriction of participating cell types. Immunology 57, 461–6.Google ScholarPubMed
Ellner, J. J. & Mahmoud, A. A. F. (1979). Killing of schistosomula of Schistosoma mansoni by normal monocytes. Journal of Immunology 123, 949–51.CrossRefGoogle Scholar
Esparza, I., Mannel, D. A., Ruppel, A., Falk, W. & Krammer, P. H. (1987). Interferon-γ and lymphotoxin or tumor necrosis factor act synergistically to induce macrophage killing of tumor cells and schistosomula of Schistosoma mansoni. Journal of Experimental Medicine 166, 589–95.CrossRefGoogle ScholarPubMed
Hamilton, T. A. & Adams, D. O. (1987). Molecular mechanisms of signal transduction in macrophages. Immunology Today 8, 151–8.CrossRefGoogle ScholarPubMed
James, S. L. (1986). Induction of protective immunity against Schistosoma mansoni by a non-living vaccine. III. Correlation of resistance with induction of activated larvacidal macrophages. Journal of Immunology 136, 3872–7.CrossRefGoogle Scholar
Jenkins, P., Dixon, J. B., Ross, G. & Cox, D. A. (1986). Echinococcus granulosus: changes in the transformational behaviour of murine lymph node cells during early infection. Annals of Tropical Medicine and Parasitology 80, 43–7.CrossRefGoogle ScholarPubMed
Joseph, M. (1982). Effector functions of phagocytic cells against helminths. Clinics in Allergy and Immunology 2, 567–96.CrossRefGoogle Scholar
Judson, D. G., Dixon, J. B., Clarkson, M. J. & Pritchard, J. (1985). Ovine hydatidosis: some immunological characteristics of the seronegative host. Parasitology 91, 349–57.CrossRefGoogle ScholarPubMed
Kelso, A., Glasebrook, A. Z., Kanagawa, O. & Brunnen, K. T. (1982). Production of macrophage activating factor by T lymphocyte clones and correlation with other lymphokine activities. Journal of Immunology 129, 550–6.CrossRefGoogle Scholar
Kowalski, J. C. & Thorson, R. E. (1972). Immunisation of laboratory mice against tetrathyridia of Mesocestoides corti (Cestoda) using a secretory and excretory antigen and a soluble somatic antigen. Journal of Parasitology 58, 732–4.CrossRefGoogle Scholar
Lammas, D. A., Mitchell, L. A. & Wakelin, D. (1987). Adoptive transfer of enhanced eosinophilia and resistance to infection in mice by an in vitro generated T-cell line specific for Mesocestoides corti larval antigen. Parasite Immunology 9, 591–601.CrossRefGoogle Scholar
Marchiondo, A. A. & Anderson, F. L. (1985). In vivo efficacy and ultrastructural effects of mitomycin C against experimental alveolar hydatid disease. Journal of Helminthology 59, 29–38.CrossRefGoogle ScholarPubMed
Mitchell, G. F. & Handman, K. (1977). Studies on immune responses to larval cestodes in mice: a simple mechanism of non-specific immunosuppression in Mesocestoides corti-infected mice. Australian Journal of Experimental Biology and Medical Science 55, 615–22.CrossRefGoogle Scholar
Morris, A. G. (1988). Interferons. Immunology Suppl. 1, 43–5.Google ScholarPubMed
Morris, A. G. & Ward, G. (1987). Production of recombinant interferons by expression in heterologous mammalian cells. In Interferons and Lymphokines: a Practical Approach, (ed. Clemens, M., Morris, A. & Gearing, A.) pp. 61–71. Oxford: I.R.L. Press.Google Scholar
Mosmann, T. R., Cherwinski, H., Bond, M. W., Giedlin, M. & Coffman, R. L. (1986). Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. Journal of Immunology 136, 2348–57.CrossRefGoogle ScholarPubMed
Olds, G. R. & Ellner, J. J. (1984). Modulation of macrophage activation and resistance by suppressor T lymphocytes in chronic murine Schistosoma mansoni infection. Journal of Immunology 133, 2720–4.CrossRefGoogle ScholarPubMed
Riley, E. M. & Dixon, J. B. (1987). Experimental Echinococcous granulosus infection in mice: immunocytochemical analysis of lymphocyte populations in local lymphoid infections during early infection. Parasitology 94, 523–32.CrossRefGoogle Scholar
Riley, E. M., Dixon, J. B., Kelly, O. F. & Cox, D. A. (1985). The immune response to Echinococcus granulosus: sequential histological observations of lymphoreticular and connective tissues during early murine infection. Journal of Comparative Pathology 95, 93–100.CrossRefGoogle ScholarPubMed
Roberts, W. K. & Vasel, A. (1982). Evidence for the identity of murine gamma interferon and macrophage activating factor. Journal of Interferon Research 2, 519–32.CrossRefGoogle ScholarPubMed
Talmadge, K. W., Frey, J. R., Kamber, M., Peck, R., Pink, R., Sinigaglia, F., Gallati, H., Walz, A. & Garotta, G. (1985). Interferon-gamma as activating factor. In Cellular and Molecular Biology of Lymphokines, (ed.Sorg, C. & Schimpl, A.), pp. 297–302. London: Academic Press.Google Scholar
Voge, M., Sogandares-Bernal, F. & Martin, J. H. (1979). Fine structure of the tegument of Mesocestoides tetrathyridia by scanning and transmission electron microscopy. Journal of Parasitology 65, 562–7.CrossRefGoogle ScholarPubMed