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A radiometric analysis of nitric oxide synthase activity in Hymenolepis diminuta

Published online by Cambridge University Press:  01 January 2000

N. B. TERENINA ,
M. V. ONUFRIEV ,
N. V. GULYAEVA ,
A. M. LINDHOLM  and
M. K. S. GUSTAFSSON
N. B. TERENINA
Affiliation:
Institute of Parasitology, Russian Academy of Sciences, Lenin Avenue 33, 117071 Moscow, Russia
M. V. ONUFRIEV
Affiliation:
Institute of Higher Nervous Activity and Neurophysiology, Department of Functional Biochemistry, Russian Academy of Sciences, 5a Butlerov Street, 117865 Moscow, Russia
N. V. GULYAEVA
Affiliation:
Institute of Higher Nervous Activity and Neurophysiology, Department of Functional Biochemistry, Russian Academy of Sciences, 5a Butlerov Street, 117865 Moscow, Russia
A. M. LINDHOLM
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
M. K. S. GUSTAFSSON
Affiliation:
Department of Biology, Åbo Akademi University, Artillerigatan 6, FIN-20520 Åbo, Finland
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Abstract

The free radical nitric oxide (NO) is a neuronal messenger which is synthesized from L-arginine and O2 by nitric oxide synthase (NOS). In the synthesis NO and L-citrulline are produced in a stoichiometric 1[ratio ]1 relation. The activity of NOS was analysed in homogenates of the rat tapeworm Hymenolepis diminuta by measuring the formation of L-[3H]citrulline after incubation with L-[3H]arginine. The nature of NOS in H. diminuta was determined by studying the effect of 3 types of NOS inhibitors: (1) L-NAME, (2) EGTA, (3) 7-nitro-indazole. All inhibitors caused a significant but not complete reduction in the formation of L-[3H]citrulline. The results are discussed against the background of nerve cells and fibres positive for NADPH-diaphorase staining in H. diminuta.

Keywords

Hymenolepis diminutanitric oxidenitric oxide synthaseL-[3H]citrullinenitric oxide synthase inhibitors
Type
Research Article
Information
Parasitology , Volume 120 , Issue 1 , January 2000 , pp. 91 - 95
Copyright
2000 Cambridge University Press

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