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Proteomic analysis of Strongyloides stercoralis L3 larvae

Published online by Cambridge University Press:  14 April 2010

A. MARCILLA ,
J. SOTILLO ,
A. PÉREZ-GARCIA ,
R. IGUAL-ADELL ,
M. LUZ VALERO ,
M. M. SÁNCHEZ-PINO ,
D. BERNAL ,
C. MUÑOZ-ANTOLÍ ,
M. TRELIS  and
R. TOLEDO
...Show all authors
A. MARCILLA*
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
J. SOTILLO
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
A. PÉREZ-GARCIA
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
R. IGUAL-ADELL
Affiliation:
Sección de Microbiología, Hospital General de Castellón, Castellón, Spain
M. LUZ VALERO
Affiliation:
Servicio de Proteómica, Centro de Investigación “Principe Felipe”, Valencia, Spain
M. M. SÁNCHEZ-PINO
Affiliation:
Servicio de Proteómica, Centro de Investigación “Principe Felipe”, Valencia, Spain
D. BERNAL
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de València, Burjassot, Valencia, Spain
C. MUÑOZ-ANTOLÍ
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
M. TRELIS
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
R. TOLEDO
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
J. G. ESTEBAN
Affiliation:
Área de Parasitología, Departament de Biologia Cel.lular i Parasitologia, Universitat de València, Burjassot, Valencia, Spain
*
*Corresponding author: Departament de Biologia Cel.lular i Parasitologia, Facultat de Farmàcia, Universitat de València, Av. V.A. Estellés, s/n, 46100 Burjassot (Valencia), Spain. Tel: +34 963544491. Fax: +34 963544769. E-mail: [email protected]
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Summary

Strongyloidiasis can be perpetuated by autoinfection with the filariform larvae L3, causing asymptomatic chronic infections and creating a population of carriers, affecting not only developing countries. So far, very little is known about the proteins that interact with the human host, and few proteins from the infective Strongyloides stercoralis L3 have been characterized. Here, we report results obtained from a proteomic analysis of the proteins from S. stercoralis L3 larvae obtained from patients. Since the genome of S. stercoralis is not yet available, we used proteomic analysis to identify 26 different proteins, 13 of them released by short digestion with trypsin, which could represent surface-associated proteins. The present work extends our knowledge of host-parasite interactions by identifying proteins that could be of interest in the development of diagnostic tools, vaccines, or treatments for a neglected disease like strongyloidiasis.

Keywords

Strongyloides stercoralis larvaehost-parasite interactionproteomics
Type
Research Article
Information
Parasitology , Volume 137 , Issue 10 , September 2010 , pp. 1577 - 1583
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Audhya, A. and Desai, A. (2008). Proteomics in Caenorhabditis elegans. Briefings in Functional Genomics and Proteomics 7, 205–210. doi:10.1093/bfgp/eln014.CrossRefGoogle ScholarPubMed
Bennuru, S., Semnani, R., Meng, Z., Ribeiro, J. M. C., Veenstra, T. D. and Nutman, T. B. (2009). Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. PLoS Neglected Tropical Diseases 3, e410. doi:10.1371/journal.pntd.0000410.Google Scholar
Bernal, D., Carpena, I., Espert, A. M., De la Rubia, J. E., Esteban, J. G., Toledo, R. and Marcilla, A. (2006). Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections. Proteomics 6, 2835–2843. doi: 10.1002/pmic.200500571.Google Scholar
Bernal, D., De la Rubia, J. E., Carrasco-Abad, A. M., Toledo, R., Mas-Coma, S. and Marcilla, A. (2004). Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica. FEBS Letters 563, 203–206. doi:10.1016/S0014-5793(04)00306-0.Google Scholar
Caffrey, C. R., Rohwer, A., Oellien, F., Marhofer, R. J., Braschi, S., Oliveira, G., McKerrow, J. H. and Selzer, P. M. (2009). A comparative chemogenic strategy to predict potential drug targets in the metazoan Schistosoma mansoni. PLoS ONE 4, e4413. doi:10.1371/journal.pone.0004413.CrossRefGoogle Scholar
Craig, H., Wastling, J. M. and Knox, D. P. (2006). A preliminary proteomic survey of the in vitro excretory/secretory products of fourth-stage larval and adult Teladorsagia circumcincta. Parasitology 132, 535–543.CrossRefGoogle ScholarPubMed
Genta, R. M. (1989). Global prevalence of strongyloidiasis: a critical review with epidemiological insights into prevention and disseminated disease. Reviews of Infectious Diseases 2, 755–766.Google Scholar
Gomez-Gallego, S., Loukas, A., Slade, L. W., Neva, F. A., Varatharajalu, R., Nutman, T. B. and Brindley, P. J. (2005). Identification of an astacin-like metallo-proteinase transcript from the infective larvae of Strongyloides stercoralis. Parasitology International 54, 123–133. doi: 10.1016/j.parint.2005.02.002.Google Scholar
Hewitson, J. P., Harcus, Y. M., Curwen, R. S., Dowle, A. A., Atmadja, A. K., Ashton, P. D., Wilson, A. and Maizels, R. M. (2008). The secretome of the filarial parasite Brugia malayi: proteomic profile of adult excretory-secretory products. Molecular and Biochemical Parasitology 160, 8–21. doi: 10.1016/j.molbiopara.2008.02.007.Google Scholar
Higon, M., Monteagudo, C., Fried, B., Esteban, J. G., Toledo, R. and Marcilla, A. (2008). Molecular cloning and characterization of Echinostoma caproni heat shock protein-70 and differential expression in the parasite derived from low- and high-compatible hosts. Parasitology 135, 1469–1477. doi:10.1017/S0031182008004927.Google Scholar
Ishihama, Y., Oda, Y., Tabata, T., Sato, T., Nagasu, T., Rappsilber, J. and Mann, M. (2005). Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein. Molecular and Cellular Proteomics 4, 1265–1272. doi:10.1074/mcp.M500061-MCP200.Google Scholar
Jaubert, S., Laffaire, J. B., Ledger, T. N., Escoubas, P., Amri, E. Z., Abad, P. and Rosso, M. (2004). Comparative analysis of two 14-3-3 homologues and their expression pattern in the root-knot nematode Meloidogyne incognita. International Journal for Parasitology 34, 873–880. doi:10.1016/j.ijpara.2004.02.008.CrossRefGoogle ScholarPubMed
Jolodar, A., Fischer, P., Bergmann, S., Büttner, D. W., Hammerschmidt, S. and Brattig, N. W. (2003). Molecular cloning of an alpha-enolase from the human filarial parasite Onchocerca volvulus that binds human plasminogen. Biochimica et Biophysica Acta 1627, 111–120. doi:10.1016/S0167-4781(03)00083-6.CrossRefGoogle ScholarPubMed
Keiser, P. B. and Nutman, T. B. (2004). Strongyloides stercoralis in the immunocompromised population. Clinical Microbiology Reviews 17, 208–217. doi: 10.1128/CMR.17.1.208-217.2004.Google Scholar
Kiel, M., Josh, P., Jones, A., Windon, R. and Hunt, P. (2007). Identification of immuno-reactive proteins from a sheep gastrointestinal nematode, Trichostrongylus colubriformis, using two-dimensional electrophoresis and mass spectrometry. International Journal for Parasitology 37, 1419–1429.CrossRefGoogle ScholarPubMed
Marcilla, A., De la Rubia, J. E., Sotillo, J., Bernal, D., Carmona, C., Villavicencio, Z., Acosta, D., Tort, J., Bornay, F. J., Esteban, J. G. and Toledo, R. (2008). Leucine aminopeptidase is an immunodominant antigen of Fasciola hepatica excretory and secretory products in human infections. Clinical and Vaccine Immunology 15, 95–100. doi:10.1128/CVI.00338-07.CrossRefGoogle ScholarPubMed
Marcilla, A., Perez-Garcia, A., Espert, A., Bernal, D., Muñoz-Antoli, C., Esteban, J. G. and Toledo, R. (2007). Echinostoma caproni: identification of enolase in excretory/secretory products, molecular cloning, and functional expression. Experimental Parasitology 117, 57–64. doi:10.1016/j.exppara.2007.03.011.Google Scholar
Marcos, L. A., Terashima, A., Dupont, H. L. and Gotuzzo, E. (2008). Strongyloides hyperinfection syndrome: an emerging global infectious disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 102, 314–318. doi:10.1016/j.trstmh.2008.01.020.Google Scholar
Mulvenna, J., Hamilton, B., Nagaraj, S., Smyth, D., Loukas, A. and Gorman, J. J. (2009). Proteomic analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, Ancylostoma caninum. Molecular and Cellular Proteomics 8, 109–121. doi:10.1074/mcp.M800206-MCP200.Google Scholar
Oleaga, A., Pérez-Sanchez, R., Pages, E., Marcos-Atxutegi, C. and Simon, F. (2009). Identification of immunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections. Molecular and Biochemical Parasitology 166, 134–141. doi: 10.1016/j.molbiopara.2009.03.005.Google Scholar
Olsen, A., van Lieshout, L., Marti, H., Polderman, T., Polman, K., Steinmann, P., Stothard, R., Thybo, S., Verweij, J. J. and Magnussen, P. (2009). Strongyloidiasis-the most neglected of the neglected diseases? Transactions of the Royal Society of Tropical Medicine and Hygiene 10, 967–972. doi: 10.1016/j.trstmh.2009.02.013.Google Scholar
Oltra, C., Igual, R., Sanchez, P., Viñals, M. J., Andreu, O., Sarrion, A. and Rodriguez-Calabuig, D. (2004). Characteristics and geographical profile of strongyloidiasis in healthcare area 11 of the Valencian community (Spain). Journal of Infection 49, 152–158. doi:10.1016/j.jinf.2004.01.016.Google Scholar
Page, A. and Johnstone, I. L. (2007). The cuticle. In The C. elegans Research Community, WormBook, doi: 10.1895/wormbook.1.138.1, http://www.wormbook.orgGoogle Scholar
Pérez-Sanchez, R., Valero, M. L., Ramajo-Hernandez, A., Siles-Lucas, M., Ramajo-Martin, V. and Oleaga, A. (2008). A proteomic approach to the identification of tegumental proteins of male and female Schistosoma bovis worms. Molecular and Biochemical Parasitology 161, 112–123. doi: 10.1074/mcp.M900045-MCP200.CrossRefGoogle Scholar
Robinson, M. W., Menon, R., Donnelly, S. M., Dalton, J. P. and Ranganathan, S. (2009). An integrated transcriptomic and proteomic analysis of the secretome of the helminth pathogen, Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. Molecular and Cellular Proteomics 8, 1891–1907. doi: 10.1074/mcp.M900045-MCP200.Google Scholar
Siddiqui, A. A. and Berk, S. L. (2001). Diagnosis of Strongyloides stercoralis infection. Clinical Infectious Diseases 33, 1040–1047. doi: 10.1086/322707.CrossRefGoogle ScholarPubMed
Sotillo, J., Valero, L., Sanchez del Pino, M. M., Fried, B., Esteban, J. G., Marcilla, A. and Toledo, R. (2008). Identification of antigenic proteins from Echinostoma caproni (Trematoda) recognized by mouse immunoglobulins M, A and G using an immunoproteomic approach. Parasitology Immunology 30, 271–279. doi: 10.1111/j.1365-3024.2007.01019.x.Google Scholar
Vadlamudi, R. S., Chi, D. S. and Krishnaswamy, G. (2006). Intestinal strongyloidiasis and hyperinfection syndrome. Clinical and Molecular Allergy 30, 4–8. doi: 10.1186/1476-7961-4-8.Google Scholar
van Hellemond, J. J., van Balkom, B. W. and Tielens, A. G. (2007). Schistosome biology and proteomics: progress and challenges. Experimental Parasitology 117, 267–274. doi: 10.1016/j.exppara.2007.05.004.Google Scholar