Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-24T05:35:28.800Z Has data issue: false hasContentIssue false
  • English
  • Français

Proteinases of Trichomonas vaginalis: antibody response in patients with urogenital trichomoniasis

Published online by Cambridge University Press:  06 April 2009

P. Bózner ,
A. Gombošová ,
M. valent ,
P. Demeš  and
J. F. Alderete
P. Bózner
Affiliation:
Institute of Microbiology and Immunology, Medical Faculty, Comenius University, Sasinkova 4, 811 08 Bratislava, Czechoslovakia
A. Gombošová
Affiliation:
Institute of Parasitology, Medical Faculty, Comenius University, Palisády 40, 811 06 Bratislava, Czechoslovakia
M. valent
Affiliation:
Institute of Parasitology, Medical Faculty, Comenius University, Palisády 40, 811 06 Bratislava, Czechoslovakia
P. Demeš
Affiliation:
Institute of Microbiology and Immunology, Medical Faculty, Comenius University, Sasinkova 4, 811 08 Bratislava, Czechoslovakia
J. F. Alderete
Affiliation:
Department of Microbiology, The University of Texas Health Science Center, San Antonio, Texas 78284–7758, USA
Get access Rights & Permissions [Opens in a new window]

Summary

Immunoprecipitation combined with electrophoresis in gelatin-polyacrylamide gels was successfully used for detection of antibodies against numerous proteinases of Trichomonas vaginalis in infected patients. The method proved to be highly specific as anti-proteinase antibodies were absent in women with negative cultivation of T. vaginalis and no history of trichomoniasis. Sera of 71% and vaginal washes of 86% patients with trichomoniasis were positive for these antibodies. In vaginal washes, but not in sera, antibodies were partly complexed with proteinases, possibly of trichomonad origin. It was also shown that serum antibodies as well as local anti-proteinase antibodies persisted for weeks after patients had been cured.

Keywords

Trichomonas vaginalistrichomoniasisproteinases
Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 387 - 391
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alderete, J. F. (1983). Antigen analysis of several pathogenic strains of Trichomonas vaginalis. Infection and Immunity 39, 1041–7.CrossRefGoogle ScholarPubMed
Alderete, J. F., Newton, E., Dennis, C. & Neale, K. A. (1991). Antibody in sera of patients infected with Trichomonas vaginalis is to trichomonad proteinases. Genitourinary Medicine 67, 331–4.Google ScholarPubMed
Arroyo, R. & Alderete, J. F. (1989). Trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. Infection and Immunity 57, 2991–7.CrossRefGoogle ScholarPubMed
Bózner, P. & Demeš, P. (1991). Proteinases of Trichomonas vaginalis and Tritrichomonas mobilensis are not exclusively of cysteine type. Parasitology 102, 113–15.CrossRefGoogle Scholar
Chappell, C. L., Dresden, M. H., Gryseels, B. & Deelder, A. M. (1990). Antibody response to Schistosoma mansoni adult worm cysteine proteinases in infected individuals. American Journal of Tropical Medicine and Hygiene 42, 335–41.CrossRefGoogle ScholarPubMed
Demeš, P., Gombošová, A., Valent, M., Jánoška, A., Fabušová, H. & Petrenko, M. (1988). Differential susceptibility of Trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus. Genitourinary Medicine 64, 176–9.Google ScholarPubMed
Diamond, L. S. (1957). The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43, 488–90.CrossRefGoogle ScholarPubMed
Garber, G. E. & Lemchuk-Favel, L. T. (1989). Characterization and purification of extracellular proteases of Trichomonas vaginalis. Canadian Journal of Microbiology 35, 903–9.CrossRefGoogle ScholarPubMed
Jaakmees, H. P., Teras, J. K., Roigas, E. M., Nigesen, U. K. & Tompel, H. J. (1966). Complement fixing antibodies in the blood sera of men infested with Trichomonas vaginalis. Wiadomości Parazytologi 12, 378–84.Google Scholar
Johnstone, A. & Thorpe, R. (1987). Immunoprecipitation. In Immunochemistry in Practice, 2nd Edn, pp. 234–40. Oxford: Blackwell Scientific Publications.Google Scholar
Kessler, S. W. (1975). Rapid isolation of antigens from cells with a staphylococcal protein A-antibody absorbent. Journal of Immunology 115, 1617.CrossRefGoogle Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.CrossRefGoogle ScholarPubMed
Lockwood, B. C., North, M. J., Scott, K. I., Bremner, A. F. & Coombs, G. H. (1987). The use of a highly sensitive electrophoretic method to compare the proteinases of trichomonads. Molecular and Biochemical Parasitology 24, 89–95.CrossRefGoogle ScholarPubMed
Neale, K. A. & Alderete, J. F. (1990). Analysis of the proteinases of representative Trichomonas vaginalis isolates. Infection and Immunity 58, 157–62.CrossRefGoogle ScholarPubMed
North, M. J., Mottram, J. C. & Coombs, G. H. (1990). Cysteine proteinases of parasitic protozoa. Parasitology Today 6, 270–5.CrossRefGoogle ScholarPubMed
Zerda, K. S., Dresden, M. H., Damian, R. T. & Chappell, C. L. (1987). Schistosoma mansoni: anti-SMw32 proteinase response in vaccinated and challenged baboons. American Journal of Tropical Medicine and Hygiene 37, 320–6.CrossRefGoogle ScholarPubMed