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Protein antigens in the cyst fluid of Taenia saginata cysticerci

Published online by Cambridge University Press:  06 April 2009

G. W. P. Joshua ,
L. J. S. Harrison  and
M. M. H. Sewell
G. W. P. Joshua
Affiliation:
University of Edinburgh, Department of Tropical Animal Health, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG
L. J. S. Harrison
Affiliation:
University of Edinburgh, Department of Tropical Animal Health, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG
M. M. H. Sewell
Affiliation:
University of Edinburgh, Department of Tropical Animal Health, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG
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Summary

Taenia saginata cyst fluid proteins from 4, 8, 12 and 16-week-old cysticerci were analysed by a combination of direct I radio-isotope labelling, immunoprecipitation using a panel of sera from infected cattle infected with T. saginata and SDS–PAGE. Protein antigens of 12, 14, 16, 20 and 26 kDa were identified in all of the cyst fluids examined. These were immunogenic and were precipitated by serum taken from cattle from 8 weeks after infection onwards and were therefore considered to be of diagnostic potential. A 185 kDa protein antigen found only in the cyst fluid of 4-week-old cysticerci and a 43 kDa protein antigen first detected in cyst fluid from 8-week-old cysticerci were also identified but were considered to be of more limited diagnostic potential due to their restricted presence. An apparently non-immunogenic 67 kDa protein, found in all the cyst fluids examined, may have been host serum albumin.

Keywords

Taenia saginata cysticercicyst fluidprotein antigens
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 463 - 467
Copyright
Copyright © Cambridge University Press 1990

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