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Protein analysis of Theileria sergenti/buffeli/orientalis piroplasms by two-dimensional polyacrylamide gel electrophoresis

Published online by Cambridge University Press:  06 April 2009

C. Sugimoto ,
S. Kawazu ,
T. Kamio  and
K. Fujisaki
C. Sugimoto
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
S. Kawazu
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
T. Kamio
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
K. Fujisaki
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305, Japan
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Extract

Proteins of the piroplasms of Theileria sergenti, T. buffeli and T. orientalis were analysed by two-dimensional polyacrylamide gel electrophoresis. Protein spot patterns of T. buffeli and T. orientalis were identical except for a few minor proteins, whereas spot patterns of two T. sergenti stocks were differentiated from those of T. buffeli and T. orientalis by a characteristic set of proteins including a major protein of molecular weight 33–34 kDa. This result indicates that Japanese T. sergenti can be phenotypically distinguishable from European and Australia Theileria species; T. orientalis and T. buffeli.

Keywords

Theileria sergentiTheileria buffeliTheileria orientalistwo-dimensional gel electrophoresis
Type
Research Article
Information
Parasitology , Volume 102 , Issue 3 , June 1991 , pp. 341 - 346
Copyright
Copyright © Cambridge University Press 1991

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