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Propagation of Cystosporogenes operophterae (Microspora), a microsporidian parasite of the winter moth, Operophtera brumata, in a Spodoptera frugiperda cell line

Published online by Cambridge University Press:  06 April 2009

Wei-Dong Xie
Wei-Dong Xie
Affiliation:
Department of Pure and Applied Biology, Imperial College, London SW7 2BB
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Summary

Cystosporogenes operophterae, a microsporidian parasite of the winter moth, Operophera brumata, was cultured in a Spodoptera frugiperda cell line. The infected cultures were achieved by addition of haemolymph from larvae infected with C. operophterae. The infection rates for the cell line ranged from 80 to 95% after incubation for 10 days at 25 or 20 °C. There was no development in cultures incubated at 30 °C. Light and electron microscopy were used to observe the development of C. operophterae in vitro.

Type
Research Article
Information
Parasitology , Volume 97 , Issue 2 , October 1988 , pp. 229 - 237
Copyright
Copyright © Cambridge University Press 1988

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