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Prevalence and molecular characterization of Sarcoptes scabiei from vicuñas (Vicugna vicugna) from Southern Peruvian Andes

Published online by Cambridge University Press:  05 November 2021

Luis A. Gomez-Puerta Open the ORCID record for Luis A. Gomez-Puerta [Opens in a new window] ,
Joel I. Pacheco ,
José M. Angulo-Tisoc ,
Wilber García ,
Hugo Castillo ,
Maria T. Lopez-Urbina  and
Armando E. Gonzalez
Luis A. Gomez-Puerta*
Affiliation:
Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Av. Circunvalacion 2800, Lima 41, Peru
Joel I. Pacheco
Affiliation:
Instituto Veterinario de Investigaciones Tropicales y de Altura, Sede Marangani, Universidad Nacional Mayor de San Marcos, Jr. Lima s/n, Cusco, Peru
José M. Angulo-Tisoc
Affiliation:
Instituto Veterinario de Investigaciones Tropicales y de Altura, Sede Marangani, Universidad Nacional Mayor de San Marcos, Jr. Lima s/n, Cusco, Peru
Wilber García
Affiliation:
Instituto Veterinario de Investigaciones Tropicales y de Altura, Sede Marangani, Universidad Nacional Mayor de San Marcos, Jr. Lima s/n, Cusco, Peru
Hugo Castillo
Affiliation:
Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Av. Circunvalacion 2800, Lima 41, Peru
Maria T. Lopez-Urbina
Affiliation:
Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Av. Circunvalacion 2800, Lima 41, Peru
Armando E. Gonzalez
Affiliation:
Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Av. Circunvalacion 2800, Lima 41, Peru
*
Author for correspondence: Luis A. Gomez-Puerta, E-mail: [email protected]
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Abstract

Sarcoptic mange is a disease caused by an infectious parasite in the vicuñas (Vicugna vicugna) from South America. Although molecular studies have provided much information about the epidemiology of this disease, this information is still unknown in vicuñas. This study determined the prevalence and molecular characterization of Sarcoptes scabiei from vicuñas from Southern Peruvian Andes. During the 2018 shearing season, 181 vicuñas were clinically evaluated for lesions compatible with mange. Sarcoptes scabiei was detected in 35 (19.3%) vicuñas, and 50 mites from 25 vicuñas were selected for molecular analyses of the mitochondrial (cox1) and nuclear (ITS2) genetic markers. Molecular analyses of the cox1 and ITS2 sequences showed an identity of 94–99% and 99.8–100% with previous S. scabiei sequences registered in the GenBank, respectively. Sequence polymorphisms were more evident in the ITS2 than in the cox1, but only the cox1 had an association with the host. Phylogenetic analysis of S. scabiei cox1 sequences from vicuñas showed a cluster with S. scabiei cox1 sequences from canids, suggesting that the origin of S. scabiei from vicuña is associated with canid mites. This research is the first molecular analysis of S. scabiei from vicuñas. Future molecular studies will be necessary to determine the species variety, geographic segregation and host–parasite adaptation for this vicuña's mite.

Keywords

cox1ITS2Sarcoptes scabieisarcoptic mangeVicugna vicugnavicuñacamelids
Type
Research Article
Information
Parasitology , Volume 149 , Issue 5 , April 2022 , pp. 581 - 586
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Acebes, P (2020) Estado de conservación de la vicuña: análisis histórico, situación actual y perspectivas de futuro. In González, BA (ed.), La Vicuña Austral. Santiago, Chile: Facultad de Ciencias Forestales y de la Conservación de la Naturaleza, Corporación Nacional Forestal y Grupo Especialista en Camélidos Sudamericanos, pp. 128–141.Google Scholar
Amer, S, El Wahab, TA, Metwaly Ael, N, Ye, J, Roellig, D, Feng, Y and Xiao, L (2014) Preliminary molecular characterizations of Sarcoptes scaibiei (Acari: Sarcoptidae) from farm animals in Egypt. PLoS ONE 9, e94705.CrossRefGoogle ScholarPubMed
Arlian, LG and Morgan, MS (2017) A review of Sarcoptes scabiei: past, present and future. Parasites and Vectors 10, 297.CrossRefGoogle ScholarPubMed
Arzamendia, Y, Neder, L, Marcoppido, G, Ortiz, F, Arce, M and Vilá, B (2012) Effect of the prevalence of ectoparasites in the behavioral patterns of wild vicuñas (Vicugna vicugna). Journal of Camelid Science 5, 105–117.Google Scholar
Astorga, F, Carver, S, Almberg, ES, Sousa, GR, Wingfield, K, Niedringhaus, KD, Van Wick, P, Rossi, L, Xie, Y, Cross, P, Angelone, S, Gortazar, C and Escobar, LE (2018) International meeting on sarcoptic mange in wildlife, June 2018, Blacksburg, Virginia, USA. Parasites and Vectors 11, 449.10.1186/s13071-018-3015-1CrossRefGoogle ScholarPubMed
Bae, M, Kim, JY, Jung, J, Cha, HH, Jeon, NY, Lee, HJ, Kim, MJ, Chang, SE and Kim, SH (2020) Diagnostic value of the molecular detection of Sarcoptes scabiei from a skin scraping in patients with suspected scabies. PLoS Neglected Tropical Diseases 14, e0008229.CrossRefGoogle ScholarPubMed
Berrilli, F, D'Amelio, S and Rossi, L (2002) Ribosomal and mitochondrial DNA sequence variation in Sarcoptes mites from different hosts and geographical regions. Parasitology Research 88, 772–777.Google ScholarPubMed
Castillo, H, Cisneros, J, Gomez-Puerta, LA and Gálvez-Durand, J (2019) Prevalencia de sarna sarcóptica en vicuñas silvestres de la comunidad campesina de San Antonio de Tanta, Lima, Perú. Revista Acadêmica Ciência Animal 17(Suppl. 1), 491–492.Google Scholar
Delaunay, P, Herisse, AL, Hasseine, L, Chiaverini, C, Tran, A, Mary, C, Del Giudice, P, Marty, P, Akhoundi, M and Hubiche, T (2020) Scabies polymerase chain reaction with standardized dry swab sampling: an easy tool for cluster diagnosis of human scabies. British Journal of Dermatology 182, 197–201.Google ScholarPubMed
Escobar, LE, Carver, S, Cross, PC, Rossi, L, Almberg, ES, Yabsley, MJ, Niedringhaus, KD, Van Wick, P, Dominguez-Villegas, E, Gakuya, F, Xie, Y, Angelone, S, Gortazar, C and Astorga, F (2021) Sarcoptic mange: an emerging panzootic in wildlife. Transboundary and Emerging Diseases. doi: 10.1111/tbed.14082.CrossRefGoogle ScholarPubMed
Fain, A (1968) Etude de la variabilite de Sarcoptes scabiei avec une revisión des Sarcoptidae. Acta Zoologica et Pathologica Antverpiensia 47, 1–196.Google Scholar
Fain, A (1994) Adaptation, specificity and host-parasite coevolution in mites (Acari). International Journal for Parasitology 24, 1273–1283.CrossRefGoogle Scholar
Fraser, TA, Charleston, M, Martin, A, Polkinghorne, A and Carver, S (2016) The emergence of sarcoptic mange in Australian wildlife: an unresolved debate. Parasites and Vectors 9, 316.CrossRefGoogle ScholarPubMed
Fraser, TA, Shao, R, Fountain-Jones, NM, Charleston, M, Martin, A, Whiteley, P, Holme, R, Carver, S and Polkinghorne, A (2017) Mitochondrial genome sequencing reveals potential origins of the scabies mite Sarcoptes scabiei infesting two iconic Australian marsupials. BMC Ecology and Evolution 17, 233.Google ScholarPubMed
Fraser, TA, Holme, R, Martin, A, Whiteley, P, Montarello, M, Raw, C, Carver, S and Polkinghorne, A (2019) Expanded molecular typing of Sarcoptes scabiei provides further evidence of disease spillover events in the epidemiology of sarcoptic mange in Australian marsupials. Journal of Wildlife Diseases 55, 231–237.10.7589/2018-04-101CrossRefGoogle ScholarPubMed
Fukuyama, S, Nishimura, T, Yotsumoto, H, Gushi, A, Tsuji, M, Kanekura, T and Matsuyama, T (2010) Diagnostic usefulness of a nested polymerase chain reaction assay for detecting Sarcoptes scabiei DNA in skin scrapings from clinically suspected scabies. British Journal of Dermatology 163, 892–894.CrossRefGoogle ScholarPubMed
Gomez-Puerta, LA, Olazabal, J, Taylor, CE, Cribillero, NG, Lopez-Urbina, MT and Gonzalez, AE (2013) Sarcoptic mange in vicuna (Vicugna vicugna) population in Peru. Veterinary Record 173, 269.CrossRefGoogle ScholarPubMed
Gomez-Puerta, LA, Alarcon, V, Pacheco, J, Franco, F, Lopez-Urbina, MT and Gonzalez, AE (2016) Molecular and morphological evidence of Taenia omissa in pumas (Puma concolor) in the Peruvian Highlands. Revista Brasileira de Parasitologia Veterinária 25, 368–373.CrossRefGoogle ScholarPubMed
Gu, X and Yang, G (2008). A study on the genetic relationship of mites in the genus Sarcoptes (Acari: Sarcoptidae) in China. International Journal of Acarology 34, 183–190.CrossRefGoogle Scholar
Korswagen, S (2016) Análisis espacial del hábitat de la vicuña en una Comunidad Campesina en la Reserva Paisajística Nor Yauyos Cochas. Espacio y Desarrollo 28, 103–128.10.18800/espacioydesarrollo.201601.005CrossRefGoogle Scholar
Krantz, GW and Walter, DE (2009) A Manual of Acarology, 3rd ed. Lubbock, TX: Texas Tech University Press.Google Scholar
Kumar, S, Stecher, G, Li, M, Knyaz, C and Tamura, K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35, 1547–1549.CrossRefGoogle ScholarPubMed
Li, CY, Sun, Y, Xie, Y, Zhou, X, Gu, XB, Lai, WM, Peng, XR and Yang, G (2018) Genetic variability of wildlife-derived Sarcoptes scabiei determined by the ribosomal ITS-2 and mitochondrial 16S genes. Experimental and Applied Acarology 76, 53–70.CrossRefGoogle ScholarPubMed
Montecino-Latorre, D, Napolitano, C, Briceño, C and Uhart, MM (2020) Sarcoptic mange: an emerging threat to Chilean wild mammals? Perspectives in Ecology and Conservation 18, 267–276.10.1016/j.pecon.2020.09.007CrossRefGoogle Scholar
Moroni, B, Angelone, S, Pérez, JM, Molinar Min, AR, Pasquetti, M, Tizzani, P, López-Olvera, JR, Valldeperes, M, Granados, JE, Lavín, S, Mentaberre, G, Camacho-Sillero, L, Martínez-Carrasco, C, Oleaga, A, Candela, M, Meneguz, PG and Rossi, L (2021) Sarcoptic mange in wild ruminants in Spain: solving the epidemiological enigma using microsatellite markers. Parasites & Vectors 14, 171.CrossRefGoogle ScholarPubMed
Navajas, M, Gutierrez, J, Bonato, O, Bolland, HR and Mapangou-Divassa, S (1994) Intraspecific diversity of the Cassava green mite Mononychellus progresivus (Acari: Tetranychidae) using comparisons of mitochondrial and nuclear ribosomal DNA sequences and cross-breeding. Experimental and Applied Acarology 18, 351–360.CrossRefGoogle ScholarPubMed
Oleaga, A, Alasaad, S, Rossi, L, Casais, R, Vicente, J, Maione, S, Soriguer, RC and Gortazar, C (2013) Genetic epidemiology of Sarcoptes scabiei in the Iberian wolf in Asturias, Spain. Veterinary Parasitology 196, 453–459.CrossRefGoogle ScholarPubMed
Peltier, SK, Brown, JD, Ternent, M, Niedringhaus, KD, Schuler, K, Bunting, EM, Kirchgessner, M and Yabsley, MJ (2017) Genetic characterization of Sarcoptes scabiei from black bears (Ursus americanus) and other hosts in the Eastern United States. Journal of Parasitology 103, 593–597.CrossRefGoogle ScholarPubMed
Pence, DB and Ueckermann, E (2002) Sarcoptic manage in wildlife. Revue Scientifique et Technique 21, 385–398.CrossRefGoogle ScholarPubMed
Ruiz, C (2016) Identificación y caracterización de la presencia de ectoparásitos y endoparásitos en vicuñas (Vicugna vicugna) en comunidades de los departamentos de La Paz y Oruro. In Facultad de Agronomía, MSc Thesis. pp. 80. Universidad Mayor de San Andrés, Bolivia.Google Scholar
Sahley, CT, Vargas, JT and Valdivia, JS (2007) Biological sustainability of live shearing of vicuna in Peru. Conservation Biology 21, 98–105.CrossRefGoogle ScholarPubMed
Tamura, K (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution 9, 678–687.Google ScholarPubMed
Verdugo, C, Espinoza, A, Moroni, M, Valderrama, R and Hernandez, C (2016) Sarcoptic mange in a South American gray fox (Chilla fox; Lycalopex griseus), Chile. Journal of Wildlife Diseases 52, 738–741.CrossRefGoogle Scholar
Walton, SF, Dougall, A, Pizzutto, S, Holt, D, Taplin, D, Arlian, LG, Morgan, M, Currie, BJ and Kemp, DJ (2004) Genetic epidemiology of Sarcoptes scabiei (Acari: Sarcoptidae) in northern Australia. International Journal for Parasitology 34, 839–849.CrossRefGoogle Scholar
Wheeler, JC (2012) South American camelids – past, present and future. Journal of Camelid Science 5, 1–24.Google Scholar
Zahler, M, Essig, A, Gothe, R and Rinder, H (1999) Molecular analyses suggest monospecificity of the genus Sarcoptes (Acari: Sarcoptidae). International Journal for Parasitology 29, 759–766.CrossRefGoogle Scholar
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