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Positive diversifying selection on Plasmodium vivax RON2 protein

Published online by Cambridge University Press:  10 February 2012

JIANXIA TANG ,
YANG DAI ,
HONGWEI ZHANG ,
RICHARD L. CULLETON ,
YAOBO LIU ,
SONG ZHAO ,
XIAOTING WANG ,
XIAOHONG GUAN ,
OSAMU KANEKO  and
YINCHANG ZHU
JIANXIA TANG
Affiliation:
School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases (JIPD), Meiyuan Yangxiang 117, Wuxi, Jiangsu, 214064, P. R. China
YANG DAI
Affiliation:
Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases (JIPD), Meiyuan Yangxiang 117, Wuxi, Jiangsu, 214064, P. R. China
HONGWEI ZHANG
Affiliation:
Department of Sanitary Inspection, Henan Center of Disease Control and Prevention, Zhengzhou, Henan, 450003, P. R. China
RICHARD L. CULLETON
Affiliation:
Malaria Unit, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
YAOBO LIU
Affiliation:
Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases (JIPD), Meiyuan Yangxiang 117, Wuxi, Jiangsu, 214064, P. R. China
SONG ZHAO
Affiliation:
Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases (JIPD), Meiyuan Yangxiang 117, Wuxi, Jiangsu, 214064, P. R. China
XIAOTING WANG
Affiliation:
Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases (JIPD), Meiyuan Yangxiang 117, Wuxi, Jiangsu, 214064, P. R. China
XIAOHONG GUAN
Affiliation:
School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China
OSAMU KANEKO
Affiliation:
Department of Protozoology, Institute of Tropical Medicine (NEKKEN) and the Global Center of Excellence Program, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
YINCHANG ZHU*
Affiliation:
School of Basic Medical Science, Nanjing Medical University, Nanjing, Jiangsu, 210029, P. R. China Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases (JIPD), Meiyuan Yangxiang 117, Wuxi, Jiangsu, 214064, P. R. China
*
*Corresponding author: Meiyuan Yangxiang 117, Wuxi, Jiangsu Province 214064, People's Republic of China. Tel: +86 510 8551 7721. Fax:+86 510 8551 0263. E-mail: [email protected]
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Summary

Plasmodium rhoptry neck protein 2 (RON2), which is released from the neck portion of the merozoite rhoptries and interacts with the microneme protein Apical Membrane Antigen 1 (AMA1), plays a crucial role in erythrocyte invasion. In this study, we sequenced the Plasmodium vivax RON2 gene from 19 P. vivax isolates collected in central China in order to establish whether this protein is under positive diversifying selection, which may occur as a result of protective host immune pressure†. In comparison with the P. vivax Sal-1 reference line, we found 10 amino acid substitutions dispersed throughout the open reading frame as well as indels caused by polymorphism in a repeat unit (21–23 repeats of (Q/E/K/N/H)(G/D)G(H/L/Y/P)G) in the second tandem repeat region located at amino acid positions 541–650. A McDonald-Kreitman test with RON2 sequences from the primate malaria parasite Plasmodium knowlesi, detected significant departure from neutrality in the PvRON2 3′ region (nucleotide positions 2668–6609). These results suggest that the PvRON2 gene has evolved under positive diversifying selection.

Keywords

Plasmodium vivaxRON2positive diversifying selectioncell invasion
Type
Research Article
Information
Parasitology , Volume 139 , Issue 6 , May 2012 , pp. 709 - 715
Copyright
Copyright © Cambridge University Press 2012

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