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Polymorphism of the gene encoding a major Polar Tube Protein PTP1 in two microsporidia of the genus Encephalitozoon

Published online by Cambridge University Press:  27 April 2001

I. PEUVEL
Affiliation:
Parasitologie Moléculaire et Cellulaire, UPESA CNRS 6023, Université Blaise Pascal, Biologie A, 63177 Aubière Cedex,France
F. DELBAC
Affiliation:
Parasitologie Moléculaire et Cellulaire, UPESA CNRS 6023, Université Blaise Pascal, Biologie A, 63177 Aubière Cedex,France
G. METENIER
Affiliation:
Parasitologie Moléculaire et Cellulaire, UPESA CNRS 6023, Université Blaise Pascal, Biologie A, 63177 Aubière Cedex,France
P. PEYRET
Affiliation:
Parasitologie Moléculaire et Cellulaire, UPESA CNRS 6023, Université Blaise Pascal, Biologie A, 63177 Aubière Cedex,France
C.P. VIVARES
Affiliation:
Parasitologie Moléculaire et Cellulaire, UPESA CNRS 6023, Université Blaise Pascal, Biologie A, 63177 Aubière Cedex,France

Abstract

Isolates of 2 microsporidian species from the genus Encephalitozoon (E. cuniculi and E. hellem) were compared by analysis of DNA amplified from a gene region encoding the repeat domain of a polar tube protein (PTP1). Sequence data obtained for 11 E. cuniculi isolates from 5 different mammalian hosts well support the existence of 3 previously designated strains. Strain type III was characterized by a lack of a 78bp repeat, producing an amplicon of reduced size. Strain type II differed from strain type I by 3 nucleotide substitutions so that AvaII digestion of the corresponding PCR products provided distinct restriction patterns. Surprisingly, the comparison of 2 human isolates of E. hellem belonging to the same rDNA ITS genotype shows a high level of heterogeneity through numerous point mutations and variation in PTP1 repeat number. Further characterization of additional E. hellem isolates based on PTP1 sequence polymorphisms should be of interest for tracing sources of infection.

Type
Research Article
Copyright
2000 Cambridge University Press

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