Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-29T02:27:52.842Z Has data issue: false hasContentIssue false
  • English
  • Français

Phylogeny of Trypanosoma (Megatrypanum) theileri and related trypanosomes reveals lineages of isolates associated with artiodactyl hosts diverging on SSU and ITS ribosomal sequences

Published online by Cambridge University Press:  03 October 2005

A. C. RODRIGUES ,
F. PAIVA ,
M. CAMPANER ,
J. R. STEVENS ,
H. A. NOYES  and
M. M. G. TEIXEIRA
A. C. RODRIGUES
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-900, Brazil
F. PAIVA
Affiliation:
Department of Veterinary Pathology, University of Mato Grosso do Sul, Campo Grande, MS, Brazil
M. CAMPANER
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-900, Brazil
J. R. STEVENS
Affiliation:
School of Biological Sciences, University of Exeter, Exeter, EX4 4PS, UK
H. A. NOYES
Affiliation:
Animal Genetics Laboratory, School of Biological Sciences, University of Liverpool, Liverpool L69 7ZD, UK
M. M. G. TEIXEIRA
Affiliation:
Department of Parasitology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, 05508-900, Brazil
Get access Rights & Permissions [Opens in a new window]

Abstract

SSU ribosomal sequences of trypanosomes from Brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic T. theileri and allied species parasitic in artiodactyls. T. theileri trypanosomes from distinct geographical regions in Brazil and from other countries were tightly clustered into the ‘clade T. theileri’ distant from the ‘T. brucei clade’ of pathogenic parasites of artiodactyls, and also distinct from trypanosomes of other mammals. The existence of this monophyletic assemblage (T. theileri clade) composed only by isolates from artiodactyl species justifies the continued recognition of the subgenus T. (Megatrypanum) with T. theileri as its type species. Phylogenies based on SSU and ITS1 ribosomal sequences produced the same branching pattern with isolates from different mammalian hosts clustered in 5 lineages: A, related to water buffalo; B, C and D, to cattle; E, to fallow deer. The pattern of host specificity allied to some congruence between host and parasite phylogenies suggested association of these trypanosomes with their respective hosts. Segregation of cattle isolates into three lineages revealed an overall geographical structure. Moreover, positioning of trypanosomes infecting tabanids in the T. theileri clade is consistent with the role of these flies as important vectors of these trypanosomes.

Keywords

Trypanosoma theileriaMegatrypanumArtiodactylacattlebuffalophylogenyevolutionribosomal genetabanid flies
Type
Research Article
Information
Parasitology , Volume 132 , Issue 2 , February 2006 , pp. 215 - 224
Copyright
2005 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aljanabi, S. M. and Martinez, I. ( 1997). Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research 25, 4692–4693.CrossRefGoogle Scholar
Bose, R., Friedhoff, K. T., Olbrich, S., Büscher, G. and Domeyer, I. ( 1987). Transmission of Trypanosoma theileri to cattle by Tabanidae. Parasitology Research 73, 421–424.CrossRefGoogle Scholar
Bose, R. and Heister, N. C. ( 1993). Development of Trypanosoma (Megatrypanum) theileri in tabanids. Journal of Eukaryotic Microbiology 40, 788–792.CrossRefGoogle Scholar
Bose, R., Petersen, K., Pospichal, H., Buchanan, N. and Tait, A. ( 1993). Characterization of Megatrypanum trypanosomes from European Cervidae. Parasitology 41, 55–61.CrossRefGoogle Scholar
Braun, U., Rogg, E., Walser, M., Nehrbass, D., Guscetti, F., Mathis, A. and Deplazes, P. ( 2002). Trypanosoma theileri in the cerebrospinal fluid and brain of a heifer with suppurative meningoencephalitis. Veterinary Record 150, 18–19.CrossRefGoogle Scholar
Dirie, M. F., Bornstein, S., Wallbanks, K. R., Molyneux, D. H. and Steen, M. ( 1990 a). Comparative studies on Megatrypanum trypanosomes from cervids. Tropical Medicine and Parasitology 41, 198–202.Google Scholar
Dirie, M. F., Bornstein, S., Wallbanks, K. R., Stiles, J. K. and Molyneux, D. H. ( 1990 b). Zymogram and life-history studies on trypanosomes of the subgenus Megatrypanum. Parasitology Research 76, 669–674.Google Scholar
Foil, L. D. ( 1989). Tabanids as vectors of disease agents. Parasitology Today 5, 88–96.CrossRefGoogle Scholar
Gibson, W. C., Stevens, J. R., Mwendia, C. M., Ngotho, J. N. and Ndung'u, J. M. ( 2001). Unravelling the phylogenetic relationships of African trypanosomes of suids. Parasitology 122, 625–631.CrossRefGoogle Scholar
Hamilton, P. B., Stevens, J. R., Gidley, J., Holz, P. and Gibson, W. C. ( 2005). A new lineage of trypanosomes from Australian vertebrates and terrestrial bloodsucking leeches (Haemadipsidae). International Journal for Parasitology 35, 431–443.CrossRefGoogle Scholar
Hamilton, P. B., Stevens, J. R., Gaunt, M. W., Gidley, J. and Gibson, W. C. ( 2004). Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA. International Journal for Parasitology 34, 1393–1404.CrossRefGoogle Scholar
Hassanin, A. and Douzery, E. J. P. ( 1999). The tribal radiation of family Bovidae (Artiodactyla) and the evolution of the mitochondrial Cytochrome b gene. Molecular Phylogenetic Evolution 13, 227–243.CrossRefGoogle Scholar
Herrera, H. M., Dávila, A. M., Norek, A., Abreu, U. G., Souza, S. S., D'Andrea, P. S. and Jansen, A. M. ( 2004). Enzootiology of Trypanosoma evansi in Pantanal, Brazil. Veterinary Parasitology 125, 263–275.CrossRefGoogle Scholar
Hoare, C. A. ( 1972). The Trypanosomes of Mammals. Blackwell, Oxford.
Hussain, K., Brodie, B., Ott, R. S. and Montealegre, F. ( 1985). Prevalence of Trypanosoma theileri in cows and fetuses at slaughters. American Journal of Veterinary Research 46, 1256–1258.Google Scholar
Kingston, N. and Morton, J. K. ( 1975). Trypanosoma cervi sp. n. from elk (Cervus canadensis) in Wyoming. Parasitology 61, 17–23.Google Scholar
Krinsky, W. L. ( 1976). Animal disease agents transmitted by horse flies and deer flies (Diptera: Tabanidae). Journal of Medical Entomology 13, 225–275.CrossRefGoogle Scholar
Krinsky, W. L. and Burgdorfer, W. ( 1976). Trypanosomes in Amblyomma americanum from Oklahoma. Journal of Parasitology 62, 824–825.CrossRefGoogle Scholar
Longan-Henfrey, L. L., Gardner, P. R. and Mahmoud, M. M. ( 1992). Animal trypanosomiasis in sub-Saharan África, In Parasitic Protozoa, Vol. II. ( ed. Kreier, J. P. and Baker, J. R.), pp. 157–276. Academic Press, London.
Maia Da Silva, F., Noyes, H. A., Campaner, M., Junqueira, A. C. V., Coura, J. R., Añez, N., Shaw, J. J., Stevens, J. R. and Teixeira, M. M. G. ( 2004). Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences. Parasitology 129, 549–561.CrossRefGoogle Scholar
Morzaria, S. P., Latif, A. A., Jongejan, F. and Walker, A. R. ( 1986). Transmission of a Trypanosoma sp to cattle by the tick Hyalomma anatolicum anatolicum. Veterinary Parasitology 19, 13–21.CrossRefGoogle Scholar
Posada, D. and Crandall, K. A. ( 1998). MODELTEST: testing the model of DNA substitution. Bioinformatics 14, 817–818.CrossRefGoogle Scholar
Martins-Neto, R. G. ( 2003). The fossil tabanids (Diptera Tabanidae): when they began to appreciate warm blood and when they began transmit diseases? Memórias do Instituto Oswaldo Cruz 98, 29–34.Google Scholar
Rodrigues, A. C., Campaner, M., Takata, C. S., Dell' Porto, A., Milder, R. V., Takeda, G. F. and Teixeira, M. M. G. ( 2003). Brazilian isolates of Trypanosoma (Megatrypanum) theileri: diagnosis and differentiation of isolates from cattle and water buffalo based on biological characteristics and randomly amplified DNA sequences. Veterinary Parasitology 116, 185–207.CrossRefGoogle Scholar
Schlafer, D. H. ( 1979). Trypanosoma theileri: A literature review and report of incidence in New York cattle. Cornell Veterinary 69, 411–425.Google Scholar
Stevens, J. R., Teixeira, M. M. G., Bingle, L. E. and Gibson, W. C. ( 1999). The taxonomic position and evolutionary relationships of Trypanosoma rangeli. International Journal for Parasitology 29, 749–757.CrossRefGoogle Scholar
Stevens, J. R., Noyes, H. A., Schofield, C. J. and Gibson, W. ( 2001). The molecular evolution of Trypanosomatidae. Advances in Parasitology 48, 1–56.CrossRefGoogle Scholar
Ventura, R. M., Paiva, F., Silva, R. M. S., Takeda, G. F., Buck, G. A. and Teixeira, M. M. G. ( 2001). Trypanosoma vivax: Characterization of the spliced-leader gene of a Brazilian stock and species-specific detection by PCR amplification of an intergenic spacer sequence. Experimental Parasitology 99, 37–38.CrossRefGoogle Scholar
Ventura, R. M., Takeda, G. F., Silva, R. A. M. S., Nunes, V. L., Buck, G. A. and Teixeira, M. M. G. ( 2002). Genetic relatedness among Trypanosoma evansi stocks by random amplification of polymorphic DNA and evaluation of synapomorphic DNA fragments for species-specific diagnosis. International Journal for Parasitology 32, 53–63.CrossRefGoogle Scholar
Wells, E. A. ( 1976). Subgenus Megatrypanum. In Biology of the Kinetoplastida, Vol. I ( ed. Lumsden, W. H. R. and Evans, D. A.), pp. 257–275. Academic Press, London.