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The persistence of component Theileria parva stocks in cattle immunized with the ‘Muguga cocktail’ live vaccine against East Coast fever in Uganda

Published online by Cambridge University Press:  10 June 2004

C. A. L. OURA ,
R. BISHOP ,
E. M. WAMPANDE ,
G. W. LUBEGA  and
A. TAIT
C. A. L. OURA
Affiliation:
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Makerere University, P.O. Box 7062, Kampala, Uganda Departmant of Veterinary Parasitology, Glasgow Veterinary School, Bearsden Road, Glasgow G611QH, UK
R. BISHOP
Affiliation:
International Livestock Research Institute, P.O. Box 30709, Nairobi, Kenya
E. M. WAMPANDE
Affiliation:
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Makerere University, P.O. Box 7062, Kampala, Uganda
G. W. LUBEGA
Affiliation:
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, Makerere University, P.O. Box 7062, Kampala, Uganda
A. TAIT
Affiliation:
Departmant of Veterinary Parasitology, Glasgow Veterinary School, Bearsden Road, Glasgow G611QH, UK
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Abstract

The ‘Muguga cocktail’ live vaccine comprises three Theileria parva stocks (Muguga, Kiambu 5 and the buffalo-derived Serengeti-transformed) and has been used extensively in Eastern, Central and Southern Africa with an infection and treatment protocol to protect cattle against East Coast fever. We report the characterization of the three component vaccine stocks using a panel of polymorphic micro-satellite and mini-satellite markers and the development of a stock-derived PCR method that distinguishes two of the vaccine stocks. These markers, with the use of a recently developed Reverse Line Blot assay, have enabled us to address four important questions in relation to vaccination. First, how closely related are the vaccine stocks, secondly do all three stocks persist post-vaccination and induce a carrier state, thirdly is there evidence for the transmission of the vaccine stocks and fourthly does vaccination prevent infection with local genotypes? The results show that Muguga and Serengeti-transformed stocks are highly related but very distinct from Kiambu 5 that persists in vaccinated cattle establishing a carrier state. No evidence was obtained for the transmission of vaccine stocks to co-grazed animals, although these animals were infected with up to 8 different T. parva genotypes showing there was a significant level of tick challenge. Some of the vaccinated animals become infected with a subset of local genotypes providing evidence for limited vaccine ‘breakthrough’.

Keywords

Theileria parva‘Muguga Cocktail’ vaccinecarrier
Type
Research Article
Information
Parasitology , Volume 129 , Issue 1 , July 2004 , pp. 27 - 42
Copyright
© 2004 Cambridge University Press

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References

REFERENCES

ALLSOPP, B., CARRINGTON, M., BAYLIS, H., SOHAL, S., DOLAN, T. & IAMS, K. (1989). Improved characterisation of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes. Molecular and Biochemical Parasitology 35, 137–148.CrossRefGoogle Scholar
BISHOP, R. P., SOHANPAL, B. K., KARIUKI, D. P., YOUNG, A. S., NENE, V., BAYLIS, H., ALLSOPP, B. A., SPOONER, P. R., DOLAN, T. T. & MORZARIA, S. P. (1992). Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction. Parasitology 104, 215–232.CrossRefGoogle Scholar
BISHOP, R., GEYSEN, D., SPOONER, P., SKILTON, R., NENE, V., DOLAN, T. & MORZARIA, S. (2001). Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle. Veterinary Parasitology 94, 227–237.CrossRefGoogle Scholar
BISHOP, R., GEYSEN, D., SKILTON, R., ODONGO, D., NENE, V., ALLSOPP, B., MBOGO, S., SPOONER, P. & MORZARIA, S. (2002). Genomic polymorphism, sexual recombination and molecular epidemiology of Theileria parva. In Theileria (ed. McKeever, D. & Dobbelaere, D. ), pp. 23–40. Kluwer Academic Press, The Netherlands.CrossRef
CONRAD, P. A., IAMS, K., BROWN, W. C., SOHANPAL, B. & OLE-MOIYOI, O. K. (1987). DNA probes detect genomic diversity in Theileria parva stocks. Molecular and Biochemical Parasitology 25, 213–226.CrossRefGoogle Scholar
CONRAD, P. A., OLE-MOIYOI, O. K., BALDWIN, C. L., DOLAN, T. T., O'CALLAGHAN, C. J., NJAMMUNGEH, R. E. G., GROOTENHUISE, J. G., STAGG, D. A., LEITCH, B. L. & YOUNG, A. S. (1989). Characterisation of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology 98, 179–188.CrossRefGoogle Scholar
GUBBELS, J. M., DE VOS, A. P., VAN DER WEIDE, M., VISERAS, J., SCHOULS, L. M., DE VRIES, E. & JONGEJAN, F. (1999). Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization. Journal of Clinical Microbiology 37, 1782–1789.Google Scholar
KARIUKI, D. P., YOUNG, A. S., MORZARIA, S. P., LESAN, A. C., MINING, S. K., OMWOYO, P., WAFULA, J. L. M. & MOLYNEUX, D. H. (1995). Theileria parva carrier state in naturally infected and artificially immunised cattle. Tropical Animal Health and Production 27, 15–25.CrossRefGoogle Scholar
KATENDE, J., MORZARIA, S., TOYE, P., SKILTON, R., NENE, V., NKONGE, C. & MUSOKE, A. (1998). An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule. Parasitology Research 84, 408–416.CrossRefGoogle Scholar
McKEEVER, D. J., TARACHA, E. L. N., INNES, E. L., MACHUGH, N. D., AWINO, E., GODDEERIS, B. M. & MORRISON, W. I. (1994). Adoptive transfer of immunity to Theileria parva in the CD8+ fraction of responding efferent lymph. Proceedings of the National Academy of Sciences, USA 91, 1959–1963.CrossRefGoogle Scholar
MORZARIA, S. P., DOLAN, T. T., NORVAL, R. A. I., BISHOP, R. P. & SPOONER, P. R. (1995). Generation and characterisation of cloned Theileria parva parasites. Parasitology 111, 39–49.CrossRefGoogle Scholar
MORZARIA, S. & WILLIAMSON, S. (1999). Live vaccines for Theileria parva: Deployment in Eastern, Central and Southern Africa. International Livestock Research Institute, Nairobi, Kenya.
OURA, C. A. L., ODONGO, D. O., LUBEGA, G. W., SPOONER, P. R., TAIT, A. & BISHOP, R. P. (2003). A panel of microsatellite and minisatellite markers for the characterization of field isolates of Theileria parva. International Journal for Parasitology 33, 1641–1653.CrossRefGoogle Scholar
OURA, C. A. L., BISHOP, R., WAMPANDE, E. M., LUBEGA, G. W. & TAIT, A. (2004). Application of a Reverse Line Blot assay to the study of haemoparasites in cattle in Uganda. International Journal for Parasitology 34/5, 603–613.CrossRefGoogle Scholar
RADLEY, D. E. (1981). Infection and treatment immunization against theieleriosis. In Proceedings of the International Conference of ILRI on Advances in the Control of Theileriosis, Nairobi, Kenya, 9–13 Febuary, 1981 (ed. Irvin, A. D., Cunningham, M. P. & Young, A. S. ), pp. 227–236. Martinus Nijhoff, The Hague.
RADLEY, D. E., BROWN, C. D. G., BURRIDGE, M. J., CUNNINGHAM, M. P., KIRIMI, I. M., PURNELL, R. E. & YOUNG, A. S. (1975 a). East Coast Fever. 1 Chemoprophylactic immunization of cattle against Theileria parva (Muguga) and five theilerial strains. Veterinary Parasitology 1, 35–41.Google Scholar
RADLEY, D. E., BROWN, C. G. D., CUNNINGHAM, M. P., KIMBER, C. D., MUSISI, F. L., PAYNE, R. E., PURNELL, R. E., STAGG, S. M. & YOUNG, A. S. (1975 b). Chemoprophylactic immunization of cattle using oxytetracycline and a combination of theilerial strains. Veterinary Parasitology 1, 51–60.Google Scholar
SKILTON, R. A., BISHOP, R. P., KATENDE, J. M., MWAURA, S. & MORZARIA, S. P. (2002). The persistence of Theileria parva infection in cattle immunized using two stocks which differ in their ability to induce a carrier state: analysis using a novel blood spot PCR assay. Parasitology 124, 265–276.CrossRefGoogle Scholar
TOYE, P. G., METZELAAR, M. J., WIJINGAARD, P. L. J., NENE, V., IAMS, K., ROOSE, J., NYANJUI, J. K., GOBRIGHT, E., MUSOKE, A. J. & CLEVERS, H. C. (1995 a). Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva. Journal of Immunology 155, 1370–1381.Google Scholar
TOYE, P., GOBRIGHT, E., NYANJUI, J., NENE, V. & BISHOP, R. (1995 b). Structure, and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PIM), an antigen of Theileria parva recognized by inhibitory monoclonal antibodies. Molecular and Biochemical Parasitology 73, 165–177.Google Scholar