Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-27T20:54:41.224Z Has data issue: false hasContentIssue false
  • English
  • Français

Pairing and aggregation of Amblosoma suwaense (Trematoda: Brachylaimidae) metacercariae in vitro and partial characterization of lipids involved in chemo-attraction

Published online by Cambridge University Press:  06 April 2009

B. Fried  and
G. A. Robinson
B. Fried
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042
G. A. Robinson
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042
Get access Rights & Permissions [Opens in a new window]

Summary

Histochemical and thin-layer chromatographic (t.l.c.) analyses were made on neutral lipids in the free (unencysted) metacercariae of Amblosoma suwaense (Brachylaimidae). As determined by t.l.c. the major neutral lipid fractions in metacercariae removed directly from Campeloma decisum snails were free sterols and sterol esters. Metacercariae incubated for 1 h at 37±1° C in sterile Locke's solution released mainly sterol esters and a lesser amount of free sterols into the medium. As determined by Oil Red O (ORO) staining, metacercariae accumulated neutral lipid in the intestinal caeca during incubation and the excretory system was ORO negative. Behavioural studies showed that metacereariae paired and aggregated in vitro and were attracted to lipophilic but not to hydrophilic worm products. Following t.l.c. preparative analysis it was demonstrated that metacercariae were attracted to sterol ester worm products but not to free sterol products.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 225 - 229
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Burren, C. H., Ehrlich, I. & Johnson, P. (1967). Excretion of lipids by the liver fluke (Fasciola hepatica L.). Lipids 2, 353–6.CrossRefGoogle ScholarPubMed
Butler, M. S. & Fried, B. (1977). Histochemical and thin layer chromatographic analyses of neutral lipids in Echinostoma revolutum metacercariae cultured in vitro. Journal of Parasitology 63, 1041–5.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957). A simple method, for the isolation and purification of total lipids from animal tissue. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle Scholar
Font, W. F. (1980). Amblosoma suwaense (Trematoda: Brachylaimidae: Leucochloridio morphinae) from Campeloma decisum in Wisconsin. Journal of Parasitology 66 (in the Press).CrossRefGoogle Scholar
Fried, B. & Appel, A. J. (1977). Excretion of lipids by Echinostoma revolutum (Trematoda) adults. Journal of Parasitology 63, 447.CrossRefGoogle ScholarPubMed
Fried, B. & Gioscia, R. M. (1976). Tentative identification of cholesterol as a chemoattractant for metacercarial pairing of Leucochloridiomorpha constantiae (Trematoda). Journal of Parasitology 62, 326–7.CrossRefGoogle Scholar
Fried, B. & Pucci, D. L. (1976). Histochemical and thin layer chromatographic analyses of neutral lipids in Leucochloridiomorpha constantiae (Trematoda) adults. International Journal for Parasitology 6, 479–82.CrossRefGoogle ScholarPubMed
Fried, B. & Roberts, T. M. (1972). Pairing of Leucochloridiomorpha constantiae (Trematoda) in vitro, in the chick and on the chorioallantois. Journal of Parasitology 58, 88–91.CrossRefGoogle ScholarPubMed
Fried, B. & Shapiro, I. L. (1975). Accumulation and excretion of neutral lipids in the metacercaria of Leucochloridiomorpha constantiae. Journal of Parasitology 61, 906–9.CrossRefGoogle ScholarPubMed
Fried, B., Tancer, R. B. & Fleming, S. J. (1980). In vitro pairing of Echinostoma revolutum (Trematoda) metacercariae and adults, and characterization of worm products involved in chemoattraction. Journal of Parasitology 66 (in the Press).CrossRefGoogle ScholarPubMed
Harris, K. R. & Cheng, T. C. (1973). Histochemical demonstration of fats associated with intestinal caeca of Leucochloridiomorpha constantiae. Transactions of the American Microscopial Society 92, 496–502.CrossRefGoogle Scholar
Kritchevsky, D. & Kirk, M. R. (1952). Detection of steroids in paper chromatography. Archives of Biochemistry and Biophysics 35, 346–51.CrossRefGoogle ScholarPubMed
Lowry, R. R. (1968). Ferric chloride spray detector for cholesterol and cholesteryl esters on thin-layer chromatograms. Journal of Lipid Research 9, 397.CrossRefGoogle ScholarPubMed
Mangold, H. K. (1969). Aliphatic lipids. In Thin Layer Chromatography (ed. Stahl, E.), pp. 363–421. New York: Springer-Verlag.CrossRefGoogle Scholar
Paul, J. (1975). Cell and Tissue Culture, 5th ed.Edinburgh: Churchill Livingstone.Google Scholar
Skipski, V. P., Smolowe, A. F., Sullivan, R. G. & Barclay, M. (1965). Separation of 1ipid classes by thin layer chromatography. Biochimica et biophysica acta 106, 386–96.CrossRefGoogle Scholar