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Organization of frozen-etched Thelohania bracteata (Strickland, 1913) (Microsporida, Nosematidae) emphasizing the fine structure of the posterior vacuole

Published online by Cambridge University Press:  06 April 2009

T. P. Liu  and
D. M. Davies
T. P. Liu
Affiliation:
Department of Biology, McMaster University, Hamilton, Canada
D. M. Davies
Affiliation:
Department of Biology, McMaster University, Hamilton, Canada
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Extract

This study examines the ultrastructure of the posterior vacuole in frozen-etched spores of Thelohania bracteata (Nosematidae), a microsporidian infecting the fat body of larval black-flies (Simuliidae). This organelle, considered important in providing intrasporal pressure for sporoplasm extrusion through the polar filament, has a double membrane with particles on its internal faces as revealed by the freeze-etching technique. The size and pattern of these particles differ from those in membranes of the polar filament and nucleus, and this difference may have functional significance. The posterior vacuole, and also the polaroplast, may originate from expanded sacs that occur in the immature spore. There is evidence from this study that there are many basic ultrastructural similarities between spores of different microsporidian species and that at least some reported differences are the result of varying techniques.

We gratefully acknowledge the freeze-etching facilities provided by the Department of Pathology, Faculty of Medicine, McMaster University. The research was supported by Grant A-130 from the National Research Council of Canada.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 2 , April 1972 , pp. 341 - 345
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Fineran, B. A. (1970). Organization of the tonoplast in frozen-etched root tips. Journal of Ultrastructure Research 33, 574–86.CrossRefGoogle ScholarPubMed
Huger, A. (1960). Electron microscope study on the cytology of a microsporidian spore by means of ultrathin sectioning. Journal of Insect Pathology 2, 84–105.Google Scholar
Ishihara, R. (1968). Some observations on the fine structure of sporoplasm discharged from spores of a microsporidian, Nosema bombycis. Journal of Invertebrate Pathology 12, 245–58.CrossRefGoogle Scholar
Kudo, R. R. & Daniels, E. W. (1963). An electron microscope study of the spore of a microsporidian, Thelohania californica. Journal of Protozoology 10, 112–20.CrossRefGoogle ScholarPubMed
Liu, T. P., Darley, J. J. & Davies, D. M. (1971). Preliminary observations on the fine structure of the pansporoblast of Thelohania bracteata (Strickland, 1913) (Microsporida, Nosematidae) as revealed by freeze-etching electron microscopy. Journal of Protozoology 18, 592–6.CrossRefGoogle ScholarPubMed
Lom, J. & Corliss, J. O. (1967). Ultrastructural observations on the development of the microsporidian protozoon Plistophora hyphessobryconis Schaperclaus. Journal of Protozoology 14, 141–52.CrossRefGoogle Scholar
Lom, J. & Vavra, J. (1963). The mode of sporoplasm extrusion in microsporidian spores. Acta Protozoologica 1, 81–9.Google Scholar
Moor, H. & Mühlethaler, K. (1963). Fine structure in frozen-etched yeast cells. Journal of Cell Biology 17, 609–28.CrossRefGoogle ScholarPubMed
Pinto da, P. Silva & Branton, D. (1970). Membrane splitting in freeze-etching. Covalently bound ferritin as a membrane marker. Journal of Cell Biology 45, 598–604.Google Scholar
Sprague, V. (1965). Nosema sp. (Microsporida, Nosematidae) in the musculature of the crab Callinectes sapidus. Journal of Protozoology 12, 66–70.CrossRefGoogle Scholar
Sprague, V. & Vernick, S. H. (1969). Light and electron microscope observations on Nosema nelsoni Sprague, 1950 (Microsporida, Nosematidae) with particular reference to its Golgi complex. Journal of Protozoology 16, 264–71.CrossRefGoogle Scholar
Sprague, V., Vernick, S. H. & Lloyd, B. J. Jr (1968). The fine structure of Nosema sp. Sprague, 1965 (Microsporida, Nosematidae) with particular reference to stages in sporogony. Journal of Invertebrate Pathology 12, 105–17.CrossRefGoogle Scholar
Stanier, J. E., Woodhouse, M. A. & Griffin, R. L. (1968). The fine structure of the spore of Nosema spelotremae, a microsporidian parasite of a Spelotrema metacercaria encysted in the crab, Carcinus maenas. Journal of Invertebrate Pathology 12, 73–82.CrossRefGoogle Scholar
Tillack, T. W. & Marchesi, V. J. (1970). Demonstration of the outer surface of freezeetched red blood cell membranes. Journal of Cell Biology 45, 649–53.CrossRefGoogle ScholarPubMed