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On the reported occurrence of an ecdysone-like steroid in the freshwater snail, Biomphalaria glabrata (Pulmonata; Basommatophora), intermediate host of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

C. J. Bayne
C. J. Bayne
Affiliation:
Museum of Zoology, University of Michigan, Ann Arbor, Michigan, U.S.A.48104
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Extract

Attempts to repeat the isolation of an ecdysone-like steroid from Biomphalaria glabrata revealed that some of the manipulations described by Muftic (1969) were physically impossible to repeat. With modified procedures no ecdysone-like steroid was found. The original publication gives scant attention to certain experimental details. Some sections of the text bear very close resemblance to earlier publications by different authors. The amount of substance claimed to have been isolated, and the long survival period claimed for Schistosoma mansoni larvae in vitro, are claims which, amongst others, seem highly unlikely a priori. All attempts to contact Muftic have proved to be futile. It is suggested that the paper is, at least in part, erroneous and that any future reference to it should clearly indicate its suspected invalidity.

Despite these results, it is considered likely that future investigations will demonstrate physiological roles for steroids in snails.

I wish to acknowledge the stimulating communications of Drs E. L. Hansen, M. Voge, R. E. Thorson, C. S. Richards, J. N. Kaplanis, G. Nace, R. Monroe, and of E. Chernin, who first brought this matter to my attention. Theanalytical steroid work could not have been done nor interpreted without the considerable time and effort willingly given by Drs F. Kohen and R. Counsell of the University of Michigan College of Pharmacy. My snail populations were supplemented with snails from the tanks of Mrs B. Caveny. Technical help was given by E. Perisho, G. Lindsay and P. LoVerde. Financial support was kindly provided by Dr J. B. Burch. This investigation was supported in part by the United States-Japan Cooperative Medical Science Program administered by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health, Department of Health, Education and Welfare, grant N.I.A.I.D. AI-7364, and in part by NSF grant GB-29315. Improvements were made to the first draft of the manuscript after it was critically read by Drs E. L. Hansen, F. Kohen, M. Voge, E. Chernin, R. E. Thorson and J. N. Kaplanis.

Type
Research Article
Information
Parasitology , Volume 64 , Issue 3 , June 1972 , pp. 501 - 509
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Anonymous (1955). The Peet-Grady Method. Soap and Chemical Specialties Bluebook 28, 249–50. 267.Google Scholar
Coles, G. C. (1970). Snail ‘metabolic hormone’ and snail parasite metabolism. Comparative Biochemistry and Physiology 34, 213–20.CrossRefGoogle ScholarPubMed
Davis, B. J. (1964). Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Science 121, 404–27.CrossRefGoogle ScholarPubMed
Davis, G. M., & Lindsay, G. K. (1967). Disc electrophoretic analysis of molluscan individuals and populations. Malacologia 5, 311–34.Google Scholar
Galbraith, M. N., & Horn, D. H. S. (1966). An insect moulting hormone from a plant. Chemical Communications 24, 905–6.Google Scholar
Hampshire, F., & Horn, D. H. S. (1966). Structure of crustecdysone, a crustacean moulting hormone. Chemical Communications no. 2, pp. 37–8.Google Scholar
Hitcho, P. J., & Thorson, R. E., (1971). Possible moulting and maturation controls in Trichinella spiralis. The Journal of Parasitology 57, 787–9.CrossRefGoogle ScholarPubMed
Horn, D. H. S., Fabbri, S., Hampshire, F., & Lowe, M. E. (1968). Isolation of crustecdysone (20 R-Hydroxyecdysone) from a crayfish (Jasus lalandei H. Milne-Edwards). Biochemical Journal 109, 399–409.CrossRefGoogle Scholar
Kaplanis, J. N., Thompson, M. J., Yamamoto, R. T., Robbins, W. E., & Louloudes, S. J. (1966 a). Ecdysones from the pupa of the Tobacco Hornworm, Manduca sexta (Johannson). Steroids 8, 605–23.CrossRefGoogle ScholarPubMed
Kaplanis, J. N., Tabor, L. A., Thompson, M. J., Robbins, W. E., & Shorting, T. J. (1966 b). Assay for ecdysone (moulting hormone) activity using the house fly, Musca domestica L. Steroids 8, 625–31.CrossRefGoogle ScholarPubMed
Lehottx, J.-G., & Sandor, T. (1970). The occurrence of steroids and steroid metabolizing enzyme systems in invertebrates. A review. Steroids 16, 141–71.Google Scholar
MacInnis, A. J. (1965). Responses of Schistosoma mansoni miracidia to chemical attractants. Journal of Parasitology 51, 731–46.CrossRefGoogle ScholarPubMed
Maldonado, J. F., & Matienzo, J. A. (1947). The development of Schistosoma mansoni in the snail intermediate host, Australorbis glabratus. Puerto Rico Journal of Public Health and Tropical Medicine 22, 331–73.Google ScholarPubMed
Muftic, M. (1969). Metamorphosis of miracidia into cercariae of Schistosoma mansoni in vitro. Parasitology 59, 365–71.CrossRefGoogle ScholarPubMed
Robbins, W. E., Kaplanis, J. N., Thompson, M. J., Shorting, T. J., & Joyner, S. C. (1970). Ecdysones and synthetic analogs: moulting hormone activity and inhibitive effects on insect growth, metamorphosis and reproduction. Steroids 16, 105–25.CrossRefGoogle Scholar
Senterfit, L. B. (1958). Immobilization of the miracidia of Schistosoma mansoni by immune sera. I. The nature of the reaction as studied in hamster sera. American Journal of Hygiene 68, 140–7.Google ScholarPubMed
Silverman, P. H., & Hansen, E. L. (1971). In vitro cultivation procedures for parasitic helminths: recent advances. Advances in Parasitology 9, 227–58.CrossRefGoogle ScholarPubMed
Takeomoto, T., Ogana, S., Nishimoto, N., & Hoffmeister, H. (1967). Steroide mit Hautungshormon-Aktivität aus Tieren und Pflanzen. Zeitschrift für Naturforschung 22, 681–2.CrossRefGoogle Scholar