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On the possible schistosomulicidal effect of macrophage-derived lysozyme

Published online by Cambridge University Press:  06 April 2009

E. Flescher ,
Y. Keisari ,
J. Lengy  and
D. Gold
E. Flescher
Affiliation:
Division of Clinical Immunology, Department of Medicine, The University of Texas Health Science Center of San Antonio, San Antonio, Texas 78284, USA
Y. Keisari
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
J. Lengy
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
D. Gold
Affiliation:
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Extract

Lysozyme secretion from macrophages of Schistosoma mansoni-infected mice was time dependent, rising significantly from the 8th week post-infection, the macrophages thereafter exhibiting very high levels (> 90%) of schistosomulicidal activity. Despite the ability of lysozyme to kill schistosomula in vitro, the concentrations required for such killing were several hundred-fold to several thousand-fold higher than those detected in the supernatants from infected-mice macrophages cultured with or without schistosomula. An in vitro lysozyme inhibitor, N, N, N-triacetyl chitobiose, did not abrogate the cytotoxic ability of macrophages from schistosome-infected mice, but an inhibitor of arginine-dependent cytotoxicity, NG-monomethyl arginine, markedly inhibited schistosomulicidal activity. Evidently, concentrations of ambient lysozyme from macrophage cultures are too low to affect schistosomula in culture, while the main schisto-somulicidal pathway in vitro seems to be arginine dependent.

Keywords

Schistosoma mansonischistosomulalysozymemacrophagesarginine
Type
Research Article
Information
Parasitology , Volume 103 , Issue 1 , August 1991 , pp. 61 - 64
Copyright
Copyright © Cambridge University Press 1991

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