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Novel trypanosome Trypanosoma gilletti sp. (Euglenozoa: Trypanosomatidae) and the extension of the host range of Trypanosoma copemani to include the koala (Phascolarctos cinereus)

Published online by Cambridge University Press:  21 July 2010

L. M. McINNES ,
J. HANGER ,
G. SIMMONS ,
S. A. REID  and
U. M. RYAN
L. M. McINNES*
Affiliation:
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia
J. HANGER
Affiliation:
The Australian Wildlife Hospital, Beerwah, Qld 4519, Australia
G. SIMMONS
Affiliation:
School of Veterinary Science, University of Queensland, St Lucia, Qld 4072, Australia
S. A. REID
Affiliation:
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia
U. M. RYAN
Affiliation:
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia
*
*Corresponding author: Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Perth, WA 6150, Australia. Tel: +61 893602495. Fax: +61 893104144. E-mail: [email protected]
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Summary

Trypanosoma irwini was previously described from koalas and we now report the finding of a second novel species, T. gilletti, as well as the extension of the host range of Trypanosoma copemani to include koalas. Phylogenetic analysis at the 18S rDNA and gGAPDH loci demonstrated that T. gilletti was genetically distinct with a genetic distance (±s.e.) at the 18S rDNA locus of 2·7±0·5% from T. copemani (wombat). At the gGAPDH locus, the genetic distance (±s.e.) of T. gilletti was 8·7±1·1% from T. copemani (wombat). Trypanosoma gilletti was detected using a nested trypanosome 18S rDNA PCR in 3/139 (∼2%) blood samples and in 2/29 (∼7%) spleen tissue samples from koalas whilst T. irwini was detected in 72/139 (∼52%) blood samples and T. copemani in 4/139 (∼3%) blood samples from koalas. In addition, naturally occurring mixed infections were noted in 2/139 (∼1·5%) of the koalas tested.

Keywords

Trypanosoma spp.Trypanosoma gillettiTrypanosoma copemaniTrypanosoma irwinikoalaPhascolarctos cinereusphylogeny18S rDNAgGAPDH
Type
Research Article
Information
Parasitology , Volume 138 , Issue 1 , January 2011 , pp. 59 - 70
Copyright
Copyright © Cambridge University Press 2010

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