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Nerolidol-loaded nanospheres prevent hepatic oxidative stress of mice infected by Trypanosoma evansi

Published online by Cambridge University Press:  17 October 2016

MATHEUS D. BALDISSERA ,
CARINE F. SOUZA ,
THIRSSA H. GRANDO ,
GEISA S. DOLCI ,
LUCIANA F. COSSETIN ,
KAREN L. S. MOREIRA ,
MARCELO L. DA VEIGA ,
MARIA IZABEL U. M. DA ROCHA ,
ALINE A. BOLIGON  and
MARLI M. A. DE CAMPOS
...Show all authors
MATHEUS D. BALDISSERA*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
CARINE F. SOUZA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
THIRSSA H. GRANDO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
GEISA S. DOLCI
Affiliation:
Department of Physiology and Pharmacology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
LUCIANA F. COSSETIN
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
KAREN L. S. MOREIRA
Affiliation:
Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
MARCELO L. DA VEIGA
Affiliation:
Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
MARIA IZABEL U. M. DA ROCHA
Affiliation:
Department of Morphology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
ALINE A. BOLIGON
Affiliation:
Laboratory of Phytochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
MARLI M. A. DE CAMPOS
Affiliation:
Laboratory of Phytochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
LENITA M. STEFANI
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil
ALEKSANDRO S. DA SILVA
Affiliation:
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil
SILVIA G. MONTEIRO*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil
*
*Corresponding author: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. E-mail: [email protected] and [email protected]
*Corresponding author: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. E-mail: [email protected] and [email protected]
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Summary

The aim of this study was to evaluate the effect of nerolidol free (N-F) and nerolidol-loaded in nanospheres (N-NS) on the hepatic antioxidant/oxidant status of mice experimentally infected by Trypanosoma evansi. In the liver it was measured: reactive oxygen species (ROS), thiobarbituric reactive acid substances (TBARS) and non-protein thiols (NPSH), catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST) and performed histopathological examination. In addition, seric levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured. Liver samples from mice infected by T. evansi showed increased (P < 0·05) ROS, TBARS, AST and ALT levels and SOD activity, and decreased NPSH levels and CAT activity (P < 0·05) compared with uninfected animals. N-NS treatment prevented (P < 0·05) ROS and TBARS increase, and increased NPSH levels, and ameliorate CAT and SOD activities on liver of infected mice. Moreover, N-NS treatment reduced (P < 0·05) AST and ALT levels, and prevented histopathological changes caused by the parasite. N-NS protected the liver from the oxidative stress caused by T. evansi, which might be due to its antioxidant properties. Nerolidol might be considered a promising therapeutic agent against oxidative stress, and nanotechnology is an encouraging approach to be explored.

Keywords

nanotechnologyliver injurytrypanosomosisantioxidant system
Type
Research Article
Information
Parasitology , Volume 144 , Issue 2 , February 2017 , pp. 148 - 157
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Copyright
Copyright © Cambridge University Press 2016 

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