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Natural occurrence of lethal aspergillosis in the cattle tick Rhipicephalus (Boophilus) microplus (Acari:Ixodidae)

Published online by Cambridge University Press:  24 October 2011

E. MIRANDA-MIRANDA ,
R. COSSIO-BAYUGAR ,
F. MARTÍNEZ-IBAÑEZ ,
R. CASASANERO-ORDUÑA  and
J. FOLCH-MALLOL
E. MIRANDA-MIRANDA*
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria del Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Boulevard Cuauhnahuac No. 8534, Colonia Progreso Jiutepec, MorelosMéxicoC.P. 62550
R. COSSIO-BAYUGAR
Affiliation:
Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria del Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Boulevard Cuauhnahuac No. 8534, Colonia Progreso Jiutepec, MorelosMéxicoC.P. 62550
F. MARTÍNEZ-IBAÑEZ
Affiliation:
Centro Nacional de Servicios de Constatación en Salud Animal SAGARPA, Carretera Federal Cuernavaca-Cuautla No. 8534, Colonia Progres, Jiutepec, MorelosMéxicoC.P. 62550
R. CASASANERO-ORDUÑA
Affiliation:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos Av. Universidad s/n., Colonia Chamilpa, Cuernavaca, Morelos, MéxicoC.P. 62210
J. FOLCH-MALLOL
Affiliation:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos Av. Universidad s/n., Colonia Chamilpa, Cuernavaca, Morelos, MéxicoC.P. 62210
*
*Corresponding author: Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria del Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Boulevard Cuauhnahuac No. 8534, Colonia Progreso, Jiutepec, MorelosMéxico, C.P. 62550. Tel: +52 777 31928 50 ext. 119. Fax: +52 777 31928 50 ext. 129. E-mail: [email protected] and [email protected]
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Summary

The purpose of this study was to describe an unreported entomopathogenic fungus that naturally infects the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Engorged female ticks, showed symptoms of fungal infection after controlled tick infestation of cattle. Infected ticks developed a distinctive dark colour, a pale mould grew over the cuticle and the ticks eventually died covered with fungal conidiophores. The responsible fungus was isolated and cultured on mycological medium and submitted to microscopic morphology, biochemical phenotyping and 18S rRNA ribotyping analyses, which identified it as aflatoxin-producing Aspergillus flavus. Spores from the cultured fungus were experimentally sprayed over healthy engorged female ticks, obtaining an 80% prevalence of experimental infection of healthy ticks and their egg masses, the larval progeny after incubation under laboratory conditions was also infected. These results demonstrate that A. flavus is the causative agent of the natural fungal disease of the cattle tick R. microplus described here.

Keywords

tick infestationbiochemical phenotyping18S rRNAribotypingaspergillosisentomopathogen
Type
Research Article
Information
Parasitology , Volume 139 , Issue 2 , February 2012 , pp. 259 - 263
Copyright
Copyright © Cambridge University Press 2011

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References

REFERENCES

Ahearm, D. G., Price, D., Simmons, R. B., Mayo, A., Zhang, S. T. and Crow, S. A. Jr. (2007). Microcycle conidiation and medusa head conidiophores of aspergilli on indoor construction materials and air filters from hospitals. Mycologia 99, 1–6. doi: 10.3852/mycologia.99.1.1.CrossRefGoogle ScholarPubMed
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389–3402.CrossRefGoogle ScholarPubMed
Barre, N., Mauleon, H., Garris, G. I. and Kermarrec, A. (1991). Predators of the tick Amblyomma variegatum (Acari: Ixodidae) in Guadeloupe, French West Indies. Experimental Applied Acarology 12, 163–170.CrossRefGoogle ScholarPubMed
Bock, R., Jackson, L., De Vos, A. and Jorgensen, W. (2004). Babesiosis of cattle. Parasitology 129 (Suppl.), S247–S269. DOI: 10.1017/S0031182004005190.CrossRefGoogle ScholarPubMed
Borneman, J. and Hartin, R. J. (2000). PCR primers that amplify fungal rRNA genes from environmental samples. Applied and Environmental Microbiology 66, 4356–4360.CrossRefGoogle ScholarPubMed
Cossio-Bayugar, R., Miranda, E. and Holman, P. J. (2005). Molecular cloning of a phospholipid-hydroperoxide glutathione peroxidase gene from the tick, Boophilus microplus (Acari: Ixodidae). Insect Biochemistry and Molecular Biology 35, 1378–1387. doi:10.1016/j.ibmb.2005.08.008.CrossRefGoogle ScholarPubMed
Estrada-Peña, A., Gonzalez, J. and Casasolas, A. (1990). The activity of Aspergillus ochraceus (fungi) on replete females of Rhipicephalus sanguineus (Acari: Ixodidae) in natural and experimental conditions. Folia Parasitologica (Praha) 37, 331–336.Google ScholarPubMed
George, J. E., Pound, J. M. and Davey, R. B. (2004). Chemical control of ticks on cattle and the resistance of these parasites to acaricides. Parasitology 129 (Suppl.), S353–S366. doi: 10.1017/S0031182003004682.CrossRefGoogle ScholarPubMed
Hedayati, M. T., Pasqualotto, A. C., Warn, P. A., Bowyer, P. and Denning, D. W. (2007). Aspergillus flavus: human pathogen, allergen and mycotoxin producer. Microbiology 153, 1677–1692. doi: 10.1099/mic.0.2007/007641-0.CrossRefGoogle ScholarPubMed
Kocan, K. M., De la Fuente, J., Blouin, E. F. and Garcia-Garcia, J. C. (2004). Anaplasma marginale (Rickettsiales: Anaplasmataceae): recent advances in defining host–pathogen adaptations of a tick-borne rickettsia. Parasitology 129 (Suppl.), S285–S300. doi: 10.1017/S0031182003004700.CrossRefGoogle ScholarPubMed
Kuhad, R. C., Kapoor, R. K. and Lal, R. (2004). Improving the yield and quality of DNA isolated from white-rot fungi. Folia Microbiologica (Praha) 49, 112–116.CrossRefGoogle ScholarPubMed
Miranda-Miranda, E., Cossio-Bayugar, R., Quezada-Delgado, M. R., Sachman-Ruiz, B. and Reynaud, E. (2010). Staphylococcus saprophyticus is a pathogen of the cattle tick Rhipicephalus (Boophilus) microplus. Biocontrol Science and Techonology 20, 1055–1067. doi: 10.1080/09583157.2010.505325.CrossRefGoogle Scholar
Miranda-Miranda, E., Cossio-Bayugar, R., Martinez-Ibañez, F. and Bautista-Garfias, C. R. (2011). Megaselia scalaris reared on Rhipicephalus (Boophilus) microplus laboratory cultures. Medical and Veterinary Entomology 25, 344–347. doi: 10.1111/j.1365-2915.2010.00930.x.CrossRefGoogle ScholarPubMed
Pirali-Kheirabadi, K., Haddadzadeh, H., Razzaghi-Abynaeh, M., Bokaie, S., Zare, R., Ghazavi, M. and Shams-Ghahfarokhi, M. (2007). Biological control of Rhipicephalus (Boophilus) annulatus by different strains of Metarhizium anisopliae, Beauveria bassiana and Lecanicillium psalliotae fungi. Parasitology Research 100, 1297–1302. doi: 10.1007/s00436-006-0410-x.CrossRefGoogle ScholarPubMed
Sambrook, J. and Russel, D. W. (2001). Molecular Cloning a Laboratory Manual, 3rd Edn.Cold Spring Harbor Laboratory Press, New Yoork, USA.Google Scholar
Samish, M., Ginsberg, H. and Glazer, I. (2004). Biological control of ticks. Parasitology 129 (Suppl.), S389–S403. doi: 10.1017/S0031182004005219.CrossRefGoogle ScholarPubMed
St. Leger, R. J., Staples, R. C. and Donald, W. R. (1993). Entomopathogenic isolates of Metarhizium anisopliae, Beauveria bassiana, and Aspergillus flavus produce multiple extracellular chitinase isozymes. Journal of Invertebrate Pathology 61, 81–84. doi:10.1006/jipa.1993.1014.CrossRefGoogle Scholar
Van der Geest, L. P. and Bruin, J. (2008). Diseases of mites and ticks: from basic pathology to microbial control- an introduction. Experimental and Applied Acarology 46, 3–6. doi: 10.1007/s10493-008-9222-2.CrossRefGoogle ScholarPubMed
Yabe, K., Ando, Y., Ito, M. and Terakado, N. (1987). Simple method for screening aflatoxin-producing molds by UV photography. Applied and Environmental Microbiology 53, 230–234.CrossRefGoogle ScholarPubMed