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Nanoencapsulation of benznidazole in calcium carbonate increases its selectivity to Trypanosoma cruzi

Published online by Cambridge University Press:  12 April 2018

Louise Donadello Tessarolo ,
Ramon Róseo Paula Pessoa Bezerra de Menezes ,
Clarissa Perdigão Mello ,
Dânya Bandeira Lima ,
Emanuel Paula Magalhães ,
Eveline Matias Bezerra ,
Francisco Adilson Matos Sales ,
Ito Liberato Barroso Neto ,
Maria de Fátima Oliveira  and
Ricardo Pires dos Santos
...Show all authors
Louise Donadello Tessarolo
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
Ramon Róseo Paula Pessoa Bezerra de Menezes
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
Clarissa Perdigão Mello
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
Dânya Bandeira Lima
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
Emanuel Paula Magalhães
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
Eveline Matias Bezerra
Affiliation:
Departamento de Ciências Naturais, Matemática e Estatística, Universidade Federal Rural do Semi-Árido, Mossoró, Rio Grande do Norte, Brasil
Francisco Adilson Matos Sales
Affiliation:
Departamento de Ensino, Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Aracati, CE, Brasil
Ito Liberato Barroso Neto
Affiliation:
Laboratório de Ciências e Tecnologia dos Materias, Departamento de Física, Universidade Federal do Ceará, Centro de Ciências, Fortaleza, CE, Brasil
Maria de Fátima Oliveira
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
Ricardo Pires dos Santos
Affiliation:
Departamento de Engenharia da Computação, Universidade Federal do Ceará, Sobral, Ceará, Brazil
Eudenilson L. Albuquerque
Affiliation:
Departmento de Biofísica e Farmacologia, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brasil
Valder Nogueira Freire
Affiliation:
Laboratório de Ciências e Tecnologia dos Materias, Departamento de Física, Universidade Federal do Ceará, Centro de Ciências, Fortaleza, CE, Brasil
Alice Maria Martins*
Affiliation:
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brasil
*
Author for correspondence: Alice Maria Martins, E-mail: [email protected]
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Abstract

Chagas disease is a public health problem, affecting about 7 million people worldwide. Benznidazole (BZN) is the main treatment option, but it has limited effectiveness and can cause severe adverse effects. Drug delivery through nanoparticles has attracted the interest of the scientific community aiming to improve therapeutic options. The aim of this study was to evaluate the cytotoxicity of benznidazole-loaded calcium carbonate nanoparticles (BZN@CaCO3) on Trypanosoma cruzi strain Y. It was observed that BZN@CaCO3 was able to reduce the viability of epimastigote, trypomastigote and amastigote forms of T. cruzi with greater potency when compared with BZN. The amount of BZN necessary to obtain the same effect was up to 25 times smaller when loaded with CaCO3 nanoparticles. Also, it was observed that BZN@CaCO3 enhanced the selectivity index. Furthermore, the cell-death mechanism induced by both BZN and BZN@CaCO3 was evaluated, indicating that both substances caused necrosis and changed mitochondrial membrane potential.

Keywords

Benznidazolecalcium carbonateChagas diseasenanoparticlesTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 145 , Issue 9 , August 2018 , pp. 1191 - 1198
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Copyright
Copyright © Cambridge University Press 2018 

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