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Multi-locus sequence typing confirms the clonality of Trichomonas gallinae isolates circulating in European finches

Published online by Cambridge University Press:  13 December 2013

PETRA GANAS ,
BARBARA JASKULSKA ,
BECKI LAWSON ,
MARKO ZADRAVEC ,
MICHAEL HESS  and
IVANA BILIC
PETRA GANAS
Affiliation:
Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria
BARBARA JASKULSKA
Affiliation:
Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria
BECKI LAWSON
Affiliation:
Institute of Zoology, Zoological Society of London, Regents Park, London NW1 4RY, UK
MARKO ZADRAVEC
Affiliation:
Veterinary Faculty, Institute for Poultry Health, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia
MICHAEL HESS
Affiliation:
Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria
IVANA BILIC*
Affiliation:
Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria
*
* Corresponding author: Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria. E-mail: [email protected]
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Summary

In recent years, Trichomonas gallinae emerged as the causative agent of an infectious disease of passerine birds in Europe leading to epidemic mortality of especially greenfinches Chloris chloris and chaffinches Fringilla coelebs. After the appearance of finch trichomonosis in the UK and Fennoscandia, the disease spread to Central Europe. Finch trichomonosis first reached Austria and Slovenia in 2012. In the present study the genetic heterogeneity of T. gallinae isolates from incidents in Austria and Slovenia were investigated and compared with British isolates. For this purpose comparative sequence analyses of the four genomic loci ITS1-5.8S-ITS2, 18S rRNA, rpb1 and Fe-hydrogenase were performed. The results corroborate that one clonal T. gallinae strain caused the emerging infectious disease within passerine birds and that the disease is continuing to spread in Europe. The same clonal strain was also found in a columbid bird from Austria. Additionally, the present study demonstrates clearly the importance of multi-locus sequence typing for discrimination of circulating T. gallinae strains.

Keywords

Trichomonas gallinaefinch trichomonosisemerging infectious diseasepasserine birdsEuropean finchessequence analysisITS1-5.8S-ITS218S rRNAFe-hydrogenaserpb1
Type
Research Article
Information
Parasitology , Volume 141 , Issue 5 , April 2014 , pp. 652 - 661
Copyright
Copyright © Cambridge University Press 2013 

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