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The molecular diagnosis of Marteilia refringens and differentiation between Marteilia strains infecting oysters and mussels based on the rDNA IGS sequence

Published online by Cambridge University Press:  14 September 2004

I. LÓPEZ-FLORES ,
R. de la HERRÁN ,
M. A. GARRIDO-RAMOS ,
J. I. NAVAS ,
C. RUIZ-REJÓN  and
M. RUIZ-REJÓN
I. LÓPEZ-FLORES
Affiliation:
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
R. de la HERRÁN
Affiliation:
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
M. A. GARRIDO-RAMOS
Affiliation:
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
J. I. NAVAS
Affiliation:
CIFPA ‘Agua del Pino’, Consejería de Agricultura y Pesca, Junta de Andalucía, 21071 Huelva, Spain
C. RUIZ-REJÓN
Affiliation:
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
M. RUIZ-REJÓN
Affiliation:
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
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Abstract

Marteilia refringens is a paramyxean parasite which infects the flat oyster Ostrea edulis and mussels (Mytilus galloprovincialis), where it has been attributed to a separate species, Marteilia maurini, by several authors. Doubts persist though as to the existence or not of two species of Marteilia in Europe. We have devised a molecular method for the diagnosis of M. refringens based on 358 bp nested-PCR of the rDNA intergene spacer (rDNA IGS) which is capable of detecting 0·5 fg of M. refringens DNA. Molecular characterization of this spacer indicates that the Marteilia parasites which infect oysters and mussels are two different strains of the same species.

Keywords

Marteilia refringensMarteilia maurinimolecular diagnosisrDNA IGSOstrea edulisMytilus galloprovincialis
Type
Research Article
Information
Parasitology , Volume 129 , Issue 4 , October 2004 , pp. 411 - 419
Copyright
2004 Cambridge University Press

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