Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-15T21:19:09.771Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization of putative Hepatozoon sp. from the sedge warbler (Acrocephalus schoenobaenus)

Published online by Cambridge University Press:  30 January 2013

ALEKSANDRA BIEDRZYCKA ,
AGNIESZKA KLOCH ,
MAGDALENA MIGALSKA  and
WOJCIECH BIELAŃSKI
ALEKSANDRA BIEDRZYCKA
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
AGNIESZKA KLOCH*
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
MAGDALENA MIGALSKA
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
WOJCIECH BIELAŃSKI
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
*
*Corresponding author. Tel: +48 608 217 909. Fax: +48 12 632 24 32. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

We characterized partial sequences of 18S rDNA from sedge warblers infected with a parasite described previously as Hepatozoon kabeeni. Prevalence was 47% in sampled birds. We detected 3 parasite haplotypes in 62 sequenced samples from infected animals. In phylogenetic analyses, 2 of the putative Hepatozoon haplotypes closely resembled Lankesterella minima and L. valsainensis. The third haplotype grouped in a wider clade composed of Caryospora and Eimeria. None of the haplotypes showed resemblance to sequences of Hepatozoon from reptiles and mammals. Molecular detection results were consistent with those from microscopy of stained blood smears, confirming that the primers indeed amplified the parasite sequences. Here we provide evidence that the avian Hepatozoon-like parasites are most likely Lankesterella, supporting the suggestion that the systematic position of avian Hepatozoon-like species needs to be revised.

Keywords

haemogregarinesHepatozoonLankesterellaavian blood parasitessedge warblerAcrocephalus schoenobaenus
Type
Research Article
Information
Parasitology , Volume 140 , Issue 6 , May 2013 , pp. 695 - 698
Copyright
Copyright © Cambridge University Press 2013

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barta, J. R., Ogedengbe, J. D., Martin, D. S. and Smith, T. G. (2012). Phylogenetic position of the Adeleorinid Coccidia (Myzozoa, Apicomplexa, Coccidia, Eucoccidiorida, Adeleorina) inferred using 18S rDNA sequences. Journal of Eukaryotic Microbiology 59, 171–180.CrossRefGoogle ScholarPubMed
Bennett, G. F., Earle, R. A. and Penzhorn, B. L. (1992 a). Ornithodoros peringueyi (Argasidae) and Xenopsylla trispinis (Siphonaptera), probable intermediate hosts of Hepatozoon atticorae of the South African Cliff Swallow, Hirundo spilodera. Canadian Journal of Zoology 70, 188–190.CrossRefGoogle Scholar
Bennett, G. F., Earle, R. A. and Peirce, M. A. (1992 b). New species of avian Hepatozoon (Apicomplexa: Haemogregarinidae) and a re-description of Hepatozoon neophrontis (Todd & Wohlbach, 1912) Wenyon, 1926. Systematic Parasitology 23, 183–193.CrossRefGoogle Scholar
Box, E. D. (1975). Exogenous stages of Isospora serini (Aragao) and Isospora canaria sp. in the canary (Serinus canarius Linnaeus). Journal of Protozoology 22, 165–169.CrossRefGoogle ScholarPubMed
Desser, S. S. (1980). An ultrastructural study of the asexual development of a presumed Isospora sp. in mononuclear, phagocytic cells of the evening grosbeak (Hesperiphona vespertina). Journal of Parasitology 66, 601–612.CrossRefGoogle ScholarPubMed
Desser, S. S. (1993). The Lankesterellidae. In Parasitic Protozoa, vol. 4 (ed. Kreier, J. P.), pp. 261–270. Academic Press, New York, USA.Google Scholar
Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Jobb, G., von Haeseler, A. and Strimmer, K. (2004). TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics. BMC Evolutionary Biology 4, 18. doi:10.1186/1471-2148-4-18.CrossRefGoogle ScholarPubMed
Kruszewicz, A. G. and Dyrcz, A. (2000). Hepatozoon kabeeni sp. (Protozoa: Apicomplexa; Hemogregarina) from the sedge warbler, Acrocephalus schoenobaenus (Aves: Passeriformes). Wiadomosci Parazytologiczne 46, 507–510.Google ScholarPubMed
Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. and Higgins, D. G. (2007). ClustalW and ClustalX version 2. Bioinformatics 23, 2947–2948.CrossRefGoogle Scholar
Levine, N. D. (1982). The genus Atoxoplasma (Protozoa: Apicomplexa). Journal of Parasitology 68, 719–723.CrossRefGoogle ScholarPubMed
Maia, J. P., Harris, D. J. and Perera, A. (2011). Molecular survey of Hepatozoon species in lizards from North Africa. Journal of Parasitology 97, 513–517.CrossRefGoogle ScholarPubMed
Merino, S., Marinez, J., Martinez-de la Puente, J., Criado-Fronelio, A., Tomas, G., Morales, J., Lobato, E. and Garcia-Fraile, S. (2006). Molecular characterization of the 18S rDNA gene of an avian Hepatozoon reveals that it is closely related to Lankesterella. Journal of Parasitology 92, 1330–1335.CrossRefGoogle ScholarPubMed
Peirce, M. A. (2005). A checklist of the valid avian species of Babesia (Apicomplexa: Piroplasmida), Haemoproteus, Laucocytozoon (Apicomplexa: Haemosporida) and Hepatozoon (Apicomplexa: Haemogregarinidae). Journal of Natural History 39, 3621–3632.CrossRefGoogle Scholar
Shurulinkov, P. and Chakarov, N. (2006). Prevalence of blood parasites in different local populations of reed warbler (Acrocephalus scirpaceus) and great reed warbler (Acrocephalus arundinaceus). Parasitology Research 99, 588–592.CrossRefGoogle ScholarPubMed
Smith, T. G. (1996). The genus Hepatozoon (Apicomplexa: Adeleina). Journal of Parasitology 82, 565–585.CrossRefGoogle ScholarPubMed
Smith, T. G. and Desser, S. S. (1997). Phylogenetic analysis of the genus Hepatozoon Moller, 1908 (Apicomplexa: Adeleorina). Systematic Parasitology 36, 213–221.CrossRefGoogle Scholar
Strimmer, K. and Rambaut, A. (2002). Inferring confidence sets of possibly misspecified gene trees. Proceedings of Royal Society of London, B 269, 137–142.CrossRefGoogle ScholarPubMed
Upton, S. J. (2000). Suborder Eimeriorina Lefer, 1911. In The Illustrated Guide to the Protozoa, 2nd Edn, Vol. 1. (ed. Lee, J. J., Leedale, G. F. and Bradbury, P.), pp. 318–339. Allen Press, Inc. Lawrence, KS, USA.Google Scholar