Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-16T05:22:16.846Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum

Published online by Cambridge University Press:  01 December 1997

B. COUVREUR ,
D. JACQUET ,
A. BOLLEN  and
D. LE RAY
B. COUVREUR
Affiliation:
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium Service de Génétique Appliquée, Université Libre de Bruxelles, rue de l'Industrie 24, B-1400 Nivelles, Belgium
D. JACQUET
Affiliation:
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium
A. BOLLEN
Affiliation:
Service de Génétique Appliquée, Université Libre de Bruxelles, rue de l'Industrie 24, B-1400 Nivelles, Belgium
D. LE RAY
Affiliation:
Laboratory for Protozoology, Prince Leopold Institute for Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium
Get access Rights & Permissions [Opens in a new window]

Abstract

Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS–PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6–9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.

Keywords

Leishmania infantumserodiagnosisimmunoelectrophoresisimmunodominant antigen
Type
Research Article
Information
Parasitology , Volume 115 , Issue 6 , December 1997 , pp. 611 - 619
Copyright
1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)