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Molecular characterization, expression and localization of a peroxiredoxin from the sheep scab mite, Psoroptes ovis

Published online by Cambridge University Press:  06 February 2009

C. M. McNAIR ,
A. J. NISBET ,
P. F. BILLINGSLEY  and
D. P. KNOX
C. M. McNAIR
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland
A. J. NISBET
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland
P. F. BILLINGSLEY
Affiliation:
Sanaria Inc., 12115 Parklawn Drive, Ste L, Rockville, MD 20852-1730, USA
D. P. KNOX*
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland
*
*Corresponding author: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland. Tel: +44 (0)131 445 5111. Fax: +44 (0)131 445 6235. E-mail: [email protected]
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Summary

The sheep scab mite, Psoroptes ovis, induces an intensely pruritic exudative dermatitis which is responsible for restlessness, loss of appetite and weight loss. Within the first 24 h of infection, there is a rapid inflammatory influx of eosinophils and apoptosis of the keratinocytes at the site of infection. The former cell type is capable of a sustained respiratory burst, toxic products of which may directly damage the mite and also contribute to lesion formation. Analysis of a P. ovis expressed sequence tag (EST) database identified a number of antioxidant enzyme-encoding sequences, including peroxiredoxin (thioredoxin peroxidase EC 1.11.1.15), all of which may help the mite endure the potentially toxic skin environment. A full length sequence encoding Po-TPx, a protein of 206 amino acids which showed high homology to a peroxiredoxin from the salivary gland of the tick Ixodes scapularis, was amplified from P. ovis cDNA. Recombinant Po-TPx was expressed in bacteria and antiserum to this protein was used to localize native Po-TPx in mite sections. Peroxiredoxin was localized, amongst other sites, to a subpharyngeal region in mite sections. The recombinant protein was recognized by sera from sheep infested with the mite suggesting that it may be secreted or excreted by the mite and interact with the host immune response.

Keywords

Psoroptes ovisantioxidantimmunityreactive oxygen species
Type
Research Article
Information
Parasitology , Volume 136 , Issue 4 , April 2009 , pp. 453 - 460
Copyright
Copyright © 2009 Cambridge University Press

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