Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-03T05:11:25.251Z Has data issue: false hasContentIssue false
  • English
  • Français

Modification and elimination of the bacterial flora in cultures of Entamoeba invadens Rodhain, 1934

Published online by Cambridge University Press:  06 April 2009

Elspeth W. McConnachie
Elspeth W. McConnachie
Affiliation:
The Molteno Institute, University of Cambridge
Get access Rights & Permissions [Opens in a new window]

Extract

1. Cysts, formed in a strain of E. invadens growing with a mixed bacterial flora, were sterilized of all but one species of bacterium (organism x), by treatment with chemicals. Strains of E. invadens derived from these cysts were prepared, growing with organism x and either Bact. coli or B. megatherium.

2. A monobacterial strain of E. invadens, growing with Bact. coli alone, was obtained by maintaining a dibacterial strain, growing with organism x and Bact. coli, for 72 days in medium containing gentian violet.

3. A monobacterial strain of E. invadens, growing with Staph. albus, was prepared from cysts, produced in the monobacterial strain of E. invadens + Bact. coli, and sterilized by treatment with chemicals.

4. An axenic strain of E. invadens was prepared by maintaining the monobacterial strain of E. invadens + Staph. albus, in saline-liver medium containing antibiotics. This axenic strain has now been maintained for over 14 months in saline containing untreated, sterile liver, but no antibiotics.

Type
Research Article
Information
Parasitology , Volume 46 , Issue 1-2 , May 1956 , pp. 117 - 129
Copyright
Copyright © Cambridge University Press 1956

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Balamuth, W. & Sandza, J. G. (1944). Simple, standardized culture medium for physiological studies on Entamoeba histolytica. Proc. Soc. Exp. Biol., N.Y., 57, 161.CrossRefGoogle Scholar
Barret, H. P. & Smith, N. M. (1923). The cultivation of an Endamoeba from the intestine of a turtle. Amer. J. Hyg. 3, 205.Google Scholar
Barret, H. P. & Smith, N. M. (1924). The cultivation of an Endamoeba from the turtle, Chelydra serpentina. Amer. J. Hyg. 4, 155.Google Scholar
Boeck, W. C. & Drbohlav, J. (1925). The cultivation of Endamoeba histolytica. Amer. J. Hyg. 5, 371.Google Scholar
Cutler, D. W. (1918). A method for the cultivation of Entamoeba histolytica. J. Path. Bact. 22, 22.CrossRefGoogle Scholar
Dobell, C. (1919). The Amoebae Living in Man. London: John Bale, Sons, and Danielsson.Google Scholar
Dobell, C. (with Neal, R. A. & Hoare, C. A.) (1952). Researches on the intestinal protozoa of monkeys and man. XII. Bacterial factors influencing the life history of Entamoeba histolytica in cultures. Parasitology, 42, 16.CrossRefGoogle ScholarPubMed
Dobell, C. & Laidlaw, P. P. (1926). On the cultivation of Entamoeba histolytica and some other entozoic amoebae. Parasitology, 18, 283.CrossRefGoogle Scholar
Dougherty, E. C. (1953). Problems of nomenclature for the growth of organisms of one species with and without associated organisms of other species. Parasitology, 42, 259.CrossRefGoogle ScholarPubMed
Lamy, L. (1948 a). Obtention simple d'une culture pure temporaire d'amibes parasites. C.R. Acad. Sci., Paris, 226, 1400.Google Scholar
Lamy, L. (1948 b). Obtention d'une culture bactériologiquement pure d'amibes parasites pathogènes (Entamoeba invadens, Rodhain) ne comportant aucune addition de germes bactériens morts, ni d'aucun extrait microbien. C.R. Acad. Sci., Paris, 226, 2021.Google Scholar
McConnachie, E. W. (1954 a). The action of amoebicidal drugs on Entamoeba invadens Rodhain, 1934 in vitro. Parasitology, 44, 132.CrossRefGoogle ScholarPubMed
McConnachie, E. W. (1954 b). The influence of environmental factors on the size of the cysts of Entamoeba invadens Rodhain, 1934. Parasitology, 44, 342.CrossRefGoogle ScholarPubMed
McConnachte, E. W. (1955). Studies on Entamoeba invadens Rodhain, 1934 in vitro, and its relationship to some other species of Entamoeba. Parasitology, 45, 452.CrossRefGoogle Scholar
Miller, M. J. (1951). A method for in vitro culture of Entamoeba invadens free from bacteria. Canadian J. Comp. Med. 15, 268.Google ScholarPubMed
Miller, M. J. (1953). Bacteria-free Entamoeba invadens. Nature, Lond., 172, 1192.CrossRefGoogle ScholarPubMed
Nakamura, M. (1953). Nutrition and physiology of Endamoeba histolytica. Bact. Rev. 17, 189.CrossRefGoogle ScholarPubMed
Neal, R. A. (1952). Experimental production of pure mixed strains of Entamoeba histolytica. Parasitology, 42, 40.CrossRefGoogle ScholarPubMed
Rao, V. Gopal (1951). Sterilization of cysts of Entamoeba histolytica by chemical disinfectants, and initiation and maintenance of pure cultures in association with single species of bacteria. Trans. R. Soc. Trop. Med. Hyg. 44, 593.Google ScholarPubMed
Rees, C. W., Baernstein, H. D., Reardon, L. V. & Phillips, L. (1953). Some interactions in vitro of Endamoeba histolytica and single species of microbial symbionts. Amer. J. Trop. Med. Hyg. 2, 1002.CrossRefGoogle ScholarPubMed
Shaffer, J. G. & Sienkiewicz, H. S. (1952). Propagation of a strain of Endamoeba histolytica in tissue-bearing culture. Science, 116, 306.CrossRefGoogle ScholarPubMed
Shaffer, J. G., Sienkiewicz, H. S. & Washington, J. E. (1953). The propagation of Endamoeba histolytica in tissue-bearing culture without accompanying bacteria or other microorganisms. Amer. J. Hyg. 57, 366.Google ScholarPubMed