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Mixed infection in the anteater Tamandua tetradactyla (Mammalia: Pilosa) from Pará State, Brazil: Trypanosoma cruzi, T. rangeli and Leishmania infantum

Published online by Cambridge University Press:  20 December 2012

VITOR ANTÔNIO L. DE ARAÚJO ,
MARIANA C. BOITÉ ,
ELISA CUPOLILLO ,
ANA MARIA JANSEN  and
ANDRÉ LUIZ R. ROQUE
VITOR ANTÔNIO L. DE ARAÚJO
Affiliation:
Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, Zip Code 21040-360, Rio de Janeiro/RJ, Brazil
MARIANA C. BOITÉ
Affiliation:
Laboratory of Leishmaniasis Research, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, Zip Code 21040-360, Rio de Janeiro/RJ, Brazil
ELISA CUPOLILLO
Affiliation:
Laboratory of Leishmaniasis Research, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, Zip Code 21040-360, Rio de Janeiro/RJ, Brazil
ANA MARIA JANSEN
Affiliation:
Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, Zip Code 21040-360, Rio de Janeiro/RJ, Brazil
ANDRÉ LUIZ R. ROQUE*
Affiliation:
Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, Zip Code 21040-360, Rio de Janeiro/RJ, Brazil
*
*Corresponding author: Laboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil 4365, Zip Code 21040-360, Rio de Janeiro/RJ, Brazil. Tel: +55 21 2562 1416. Fax: +55 21 2562 1609. E-mail: [email protected]
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Summary

Some Trypanosoma and Leishmania species are multi-host parasites whose distribution overlaps in several parts of the Brazilian Amazon basin. Despite being a common trait among wild mammals, mixed infections and their consequences for the host's health and parasite transmission are still a poorly known phenomenon. Here we describe a triple mixed infection – Trypanosoma cruzi, T. rangeli and Leishmania infantum – in a bone marrow sample from an anteater Tamandua tetradactyla captured in a house backyard from the endemic Abaetetuba municipality in the Amazon basin. T. cruzi was also isolated from blood samples. The mini-exon multiplex PCR characterization detected the infection by T. rangeli and T. cruzi (TcI genotype), while L. infantum infection was confirmed by an ITS-PCR followed by amplicon sequencing. This is the first description of T. rangeli isolation from bone marrow and the first report of L. infantum infection in xenarthrans. The implications of this finding are discussed considering the influence of mixed infections in the role of this mammal species as a putative reservoir host of these 3 trypanosomatid species.

Keywords

Tamandua tetradactylaTrypanosoma cruziTrypanosoma rangeliLeishmania infantumbone marrowreservoirs
Type
Research Article
Information
Parasitology , Volume 140 , Issue 4 , April 2013 , pp. 455 - 460
Copyright
Copyright © Cambridge University Press 2012

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