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Mapping of the conserved antigenic domains shared between potato apyrase and parasite ATP diphosphohydrolases: potential application in human parasitic diseases

Published online by Cambridge University Press:  04 July 2008

P. FARIA-PINTO ,
F. A. REZENDE-SOARES ,
A. M. MOLICA ,
M. A. MONTESANO ,
M. J. MARQUES ,
M. O. C. ROCHA ,
J. A. S. GOMES ,
M. J. ENK ,
R. CORREA-OLIVEIRA  and
P. M. Z. COELHO
...Show all authors
P. FARIA-PINTO
Affiliation:
Departamento de Bioquímica/ICB, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, and
F. A. REZENDE-SOARES
Affiliation:
Departamento de Bioquímica/ICB, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil
A. M. MOLICA
Affiliation:
Departamento de Bioquímica/ICB, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
M. A. MONTESANO
Affiliation:
Departamento de Bioquímica/ICB, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil
M. J. MARQUES
Affiliation:
Departamento de Ciências Biológicas, Universidade Federal de Alfenas, Alfenas, MG, Brazil
M. O. C. ROCHA
Affiliation:
Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
J. A. S. GOMES
Affiliation:
Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, and
M. J. ENK
Affiliation:
Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, and
R. CORREA-OLIVEIRA
Affiliation:
Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, and
P. M. Z. COELHO
Affiliation:
Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, and Sta Casa de Belo Horizonte, Belo Horizonte, MG, Brazil
S. M. NETO
Affiliation:
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil
O. L. FRANCO
Affiliation:
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil
E. G. VASCONCELOS*
Affiliation:
Departamento de Bioquímica/ICB, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil
*
*Corresponding author: Laboratório de Estrutura e Função de Proteínas, Departamento de Bioquímica, ICB, Universidade Federal de Juiz de Fora, Campus Universitário, Bairro Cidade Universitária, 36036-330, Juiz de Fora, MG, Brazil. Tel: +55 32 3229 3217. Fax: +55 32 3229 3216. E-mail: [email protected]; [email protected]
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Summary

Evolutionary and closer structural relationships are demonstrated by phylogenetic analysis, peptide prediction and molecular modelling between Solanum tuberosum apyrase, Schistosoma mansoni SmATPase 2 and Leishmania braziliensis NDPase. Specific protein domains are suggested to be potentially involved in the immune response, and also seem to be conserved during host and parasite co-evolution. Significant IgG antibody reactivity was observed in sera from patients with American cutaneous leishmaniasis (ACL) and schistosomiasis using potato apyrase as antigen in ELISA. S. mansoni adult worm or egg, L. braziliensis promastigote (Lb) and Trypanosoma cruzi epimastigote (EPI) have ATP diphosphohydrolases, and antigenic preparations of them were evaluated. In ACL patients, IgG seropositivity was about 43% and 90% for Lb and potato apyrase, respectively, while IgM was lower (<19%) for both. In schistosomiasis patients IgM (>40%) or IgG (100%) seropositivity for both soluble egg (SEA) and adult worm (SWAP) antigens was higher than that found for potato apyrase (IgM=10%; IgG=39%). In Chagas disease, IgG seropositivity for EPI and potato apyrase was 97% and 17%, respectively, while the IgM was low (3%) for both antigens. The study of the conserved domains from both parasite proteins and potato apyrase could lead to the development of new drug targets or molecular markers.

Keywords

ATP diphosphohydrolaseapyraseNTPDaseLeishmania braziliensisSchistosoma mansoniTrypanosoma cruziSolanum tuberosumantibodymolecular modellingphylogenetic
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 8 , July 2008 , pp. 943 - 953
Copyright
Copyright © 2008 Cambridge University Press

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