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Major acid endopeptidases of the blood-feeding monogenean Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae)

Published online by Cambridge University Press:  18 February 2016

LUCIE JEDLIČKOVÁ ,
HANA DVOŘÁKOVÁ ,
MARTIN KAŠNÝ ,
JANA ILGOVÁ ,
DAVID POTĚŠIL ,
ZBYNĚK ZDRÁHAL  and
LIBOR MIKEŠ
LUCIE JEDLIČKOVÁ
Affiliation:
Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic
HANA DVOŘÁKOVÁ
Affiliation:
Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic
MARTIN KAŠNÝ
Affiliation:
Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic Department of Botany and Zoology, Faculty of Science, Masaryk University, Kamenice 753/5 (A31), 625 00 Brno, Czech Republic
JANA ILGOVÁ
Affiliation:
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kamenice 753/5 (A31), 625 00 Brno, Czech Republic
DAVID POTĚŠIL
Affiliation:
RG Proteomics, Central European Institute of Technology, Masaryk University, Brno, Kamenice 753/5 (A26), 625 00 Brno Bohunice, Czech Republic
ZBYNĚK ZDRÁHAL
Affiliation:
RG Proteomics, Central European Institute of Technology, Masaryk University, Brno, Kamenice 753/5 (A26), 625 00 Brno Bohunice, Czech Republic
LIBOR MIKEŠ*
Affiliation:
Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic
*
* Corresponding author. Department of Parasitology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic. E-mail: [email protected]
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Summary

In parasitic flatworms, acid endopeptidases are involved in crucial processes, including digestion, invasion, interactions with the host immune system, etc. In haematophagous monogeneans, however, no solid information has been available about the occurrence of these enzymes. Here we aimed to identify major cysteine and aspartic endopeptidase activities in Eudiplozoon nipponicum, an invasive haematophagous parasite of common carp. Employing biochemical, proteomic and molecular tools, we found that cysteine peptidase activities prevailed in soluble protein extracts and excretory/secretory products (ESP) of E. nipponicum; the major part was cathepsin L-like in nature supplemented with cathepsin B-like activity. Significant activity of the aspartic cathepsin D also occurred in soluble protein extracts. The degradation of haemoglobin in the presence of ESP and worm protein extracts was completely inhibited by a combination of cysteine and aspartic peptidase inhibitors, and diminished by particular cathepsin L, B and D inhibitors. Mass spectrometry revealed several tryptic peptides in ESP matching to two translated sequences of cathepsin L genes, which were amplified from cDNA of E. nipponicum and bioinformatically annotated. The dominance of cysteine peptidases of cathepsin L type in E. nipponicum resembles the situation in, e.g. fasciolid trematodes.

Keywords

cysteine peptidaseaspartic peptidaseproteasehaematophagous monogeneacathepsin Lcathepsin Bcathepsin Dfish parasitecommon carp
Type
Research Article
Information
Parasitology , Volume 143 , Issue 4 , April 2016 , pp. 494 - 506
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Copyright
Copyright © Cambridge University Press 2016 

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