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Ligula intestinalis (L.) (Cestoda: Pseudophyllidea): polarization of cyprinid leucocytes as an indicator of host-and parasite-derived chemoattractants

Published online by Cambridge University Press:  06 April 2009

M. J. Taylor  and
D. Hoole
M. J. Taylor
Affiliation:
Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
D. Hoole
Affiliation:
Parasitology Research Group, Centre for Applied Entomology and Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK
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Summary

Freshly isolated pronephric leucocytes from roach, Rutilus rutilus and gudgeon, Gobio gobio were exposed to extracts of plerocercoids of Ligula intestinalis from these two cyprinid fish. Addition of the extracts or an increase in incubation temperature from 10 to 20 °C induced polarization of neutrophils and L1 granulocytes. Cells were transformed from their typical spherical shape to elongate forms possessing a ruffled leading edge. Extracts obtained from gudgeon-Ligula stimulated polarization of both roach and gudgeon leucocytes at 10 and 20 °C. In contrast, extracts from roach-Ligula, whilst having little effect at 10 °C, suppressed temperature-induced polarization of leucocytes at 20 °C. Addition of serum to all the essays enhanced polarization and abolished the roach-Ligula-induced suppression. It is suggested that leucocyte chemoattractants are present in Ligula from roach and gudgeon and only parasites from the former host contain an inhibitor of polarization. In addition, host-derived factors possibly complement, may be involved in leucocyte chemoattraction.

Keywords

Ligula intestinalisfish leucocyte polarizationchemoattractants
Type
Research Article
Information
Parasitology , Volume 107 , Issue 4 , November 1993 , pp. 433 - 440
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Berezantsev, Y. A. & Oparin, E. N. (1976). Telergones of cestode larvae that inhibit chemotaxis in host leucocytes. Doklady Akademii nauk SSSR 226, 1236–9.Google Scholar
Haston, W. S. & Shields, J. M. (1985). Neutrophil leucocyte chemotaxis: a simplified assay for measuring polarising responses to chemotactic factors. Journal of Immunological Methods 72, 229–37.Google Scholar
Hoole, D. & Arme, C. (1982). Ultrastructural studies on the cellular response of roach, Rutilus rutilus L., to the plerocercoid larva of the pseudophyllidean cestode, Ligula intestinalis. Journal of Fish Diseases 5, 131–44.Google Scholar
Hoole, D. & Arme, C. (1983 a). Ligula intestinalis (Cestoda: Pseudophyllidea): an ultrastructural study on the cellular response of roach fry, Rutilus rutilus. International Journal for Parasitology 13, 359–63.CrossRefGoogle Scholar
Hoole, D. & Arme, C. (1983 b). Ultrastructural studies on the cellular response of fish hosts following experimental infection with the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea). Parasitology 87, 139–49.CrossRefGoogle Scholar
Hoole, D. & Arme, C. (1985). The in vitro culture and tegumental dynamics of the plerocercoid of Ligula intestinalis (Cestoda: Pseudophyllidea). International Journal for Parasitology 15, 606–15.Google Scholar
Hunt, T. C. & Rowley, A. F. (1986 a). Leukotriene B4 induces enhanced migration of fish leucocytes in vitro. Immunology 59, 563–8.Google ScholarPubMed
Hunt, T. C. & Rowley, A. F. (1986 b). Preliminary studies on the chemotactic potential of dogfish (Scyliorhinus canicula) leucocyte using the bipolar shape formation assay. Veterinary Immunology and Immunopathology 12, 75–82.Google Scholar
James, S. & Sher, A. (1980). Immune mechanisms that stimulate mouse leukocyte migration in response to schistosomula of Schistosoma mansoni. Journal of Immunology 124, 1837–44.CrossRefGoogle ScholarPubMed
King, C. H., Spagnuolo, P. J. & Greene, B. M. (1983). Chemotaxis of human granulocytes towards microfilariae of Onchocerca volvulus. Parasite Immunology 5, 217–24.Google Scholar
Leid, R. W. (1988). Parasites and complement. In Advances in Parasitology, Vol. 27 (ed. Baker, J. R. & Müller, R.), pp. 131–58. London: Academic Press.Google Scholar
Leid, R. W., Grant, R. F. & Suquet, C. M. (1987 a). Inhibition of neutrophil chemotaxis and chemokinesis by a Taenia taeniaeformis proteinase inhibitor, taeniaestatin. Parasite Immunology 9, 195–204.Google Scholar
Leid, R. W., Grant, R. F. & Suquet, C. M. (1987 b). Inhibition of neutrophil aggregation by taeniaestatin, a cestode proteinase inhibitor. International Journal for Parasitology 17, 1349–53.Google Scholar
MacArthur, J. I., Thompson, A. W. & Fletcher, T. C. (1985). Aspects of leucocyte migration in the plaice, Pleuronectes platessa L. Journal of Fish Biology 27, 667–76.Google Scholar
Nash, K. A., Fletcher, T. C. & Thompson, A. W. (1986). Migration of fish leucocytes in vitro: the effect of factors which may be involved in mediating inflammation. Veterinary Immunology and Immunopathology 12, 83–92.CrossRefGoogle ScholarPubMed
Obenauf, S. D. & Hyder-Smith, S. (1985). Chemotaxis of nurse shark leukocytes. Developmental and Comparative Immunology 9, 221–30.CrossRefGoogle ScholarPubMed
Sharp, G. J. E., Pike, A. W. & Secombes, C. J. (1991). Leucocyte migration in rainbow trout (Oncorhynchus mykiss [Walbaum]): Optimization of migration conditions and responses to host and pathogen (Diphyllobothrium dendriticum [Nitzsch]) derived chemoattractants. Developmental and Comparative Immunology 15, 295–305.Google Scholar
Shields, J. M. & Haston, W. S. (1985). Behaviour of neutrophil leucocytes in uniform concentrations of chemotactic factors: contraction waves, cell polarity and persistence. Journal of Cell Science 74, 75–93.CrossRefGoogle ScholarPubMed
Suzuki, Y. (1986). Neutrophil chemotactic factor in eel blood plasma. Bulletin of the Japanese Society of Scientific Fisheries 52, 811–16.CrossRefGoogle Scholar
Taylor, M. J. & Hoole, D. (1989). Ligula intestinalis (L.) (Cestoda: Pseudophyllidea): plerocercoid-induced changes in the spleen and pronephros of roach, Rutilus rutilus (L.), and gudgeon, Gobio gobio (L.). Journal of Fish Biology 34, 583–96.Google Scholar
Weeks, B. A., Sommer, S. R. & Dalton, H. P. (1988). Chemotactic response of fish macrophages to Legionella pneumophila: correlation with pathogenicity. Diseases of Aquatic Organisms 5, 35–8.Google Scholar
Wilkinson, P. C. & Haston, W. S. (1988). Chemotaxis: an overview. In Immunological Techniques. Part L, Chemotaxis and Inflammation. Methods in Enzymology, Vol. 162 (ed. Di Sabato, G.), pp. 3–16. London: Academic Press.Google Scholar
Wood, B. P. & Matthews, R. A. (1987). The immune response of the thick-lipped grey mullet, Chelon labrosus, to metacercarial infections of Cryptocotyle lingua. Journal of Fish Biology 31, 175–84Google Scholar
Zigmond, S. M., Levitsky, H. L. & Kreel, B. J. (1981). Cell polarity: an examination of its behavioural expression and its consequences for polymorphonuclear leukocyte chemotaxis. Journal of Cell Biology 89, 585–92.Google Scholar