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Light and electron microscopical observations of the effects of high-density lipoprotein on growth of Plasmodium falciparum in vitro

Published online by Cambridge University Press:  13 May 2004

H. IMRIE ,
D. J. P. FERGUSON ,
M. CARTER ,
J. DRAIN ,
A. SCHIFLETT ,
S. L. HAJDUK  and
K. P. DAY
H. IMRIE
Affiliation:
Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford OX1 3SY, UK
D. J. P. FERGUSON
Affiliation:
Department of Pathology and Bacteriology, John Radcliffe Hospital, Oxford OX39DU, UK
M. CARTER
Affiliation:
Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford OX1 3SY, UK
J. DRAIN
Affiliation:
Department of Biochemistry and Molecular Genetics, School of Medicine, University of Alabama at Birmingham, Birmingham AL, 35294, USA
A. SCHIFLETT
Affiliation:
Marine Biological Laboratory, Woods Hole, MA 02543, USA
S. L. HAJDUK
Affiliation:
Marine Biological Laboratory, Woods Hole, MA 02543, USA
K. P. DAY
Affiliation:
Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford OX1 3SY, UK
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Abstract

Human serum high-density lipoprotein (HDL) is necessary and sufficient for the short-term maintenance of Plasmodium falciparum in in vitro culture. However, at high concentrations it is toxic to the parasite. A heat-labile component is apparently responsible for the stage-specific toxicity to parasites within infected erythrocytes 12–42 h after invasion, i.e. during trophozoite maturation. The effects of HDL on parasite metabolism (as determined by nucleic acid synthesis) are evident at about 30 h after invasion. Parasites treated with HDL show gross abnormalities by light and electron microscopy.

Keywords

Plasmodium falciparumhigh density lipoprotein
Type
Research Article
Information
Parasitology , Volume 128 , Issue 6 , June 2004 , pp. 577 - 584
Copyright
2004 Cambridge University Press

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