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A larval paralysis assay for the detection of thiabendazole resistance in trichostrongyles

Published online by Cambridge University Press:  06 April 2009

I. A. Sutherland  and
D. L. Lee
I. A. Sutherland
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT
D. L. Lee
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT
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Summary

Infective-stage larvae of trichostrongyle nematodes, either resistant or susceptible to thiabendazole (TBZ), were incubated in eserine, an acetylcholinesterase inhibitor. Paralysis occurred in larvae treated with eserine but significant differences were observed in the percentage of larva immobilized between TBZ-resistant and TBZ-susceptible strains of the nematodes. These differences are probably related to the presence of higher levels of acetylcholinesterase in the TBZ-resistant strains than in the susceptible strains. This could be used as a rapid and inexpensive method of detecting resistance to TBZ in trichostrongyles.

Keywords

anthelmintic-resistancebenzimidazolesHaemonchus contortusnematodeOstertagia circumcinctaTrichostrongylus colubriformis
Type
Research Article
Information
Parasitology , Volume 100 , Issue 1 , February 1990 , pp. 131 - 135
Copyright
Copyright © Cambridge University Press 1990

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