Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-24T04:43:45.788Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation of Leishmania (Viannia) braziliensis glycolipid antigens and their reactivity with mAb SST-1, specific for parasites of Viannia subgenus

Published online by Cambridge University Press:  15 August 2005

T. G. V. SILVEIRA ,
K. A. G. YONEYAMA ,
H. K. TAKAHASHI  and
A. H. STRAUS
T. G. V. SILVEIRA
Affiliation:
Department of Biochemistry,Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil Departamento de Análises Clínicas, Universidade Estadual de Maringá, Maringá, PR, 87020-900, Brazil
K. A. G. YONEYAMA
Affiliation:
Department of Biochemistry,Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
H. K. TAKAHASHI
Affiliation:
Department of Biochemistry,Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
A. H. STRAUS
Affiliation:
Department of Biochemistry,Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, São Paulo, SP, 04023-900, Brazil
Get access Rights & Permissions [Opens in a new window]

Abstract

Specific glycolipids (GLs) from Leishmania (Viannia) braziliensis promastigotes were isolated and purified. A monoclonal antibody directed to carbohydrate epitopes of these GLs was produced. mAb SST-1 recognizes a low molecular weight GL as established by solid-phase radioimmunoassay and HPTLC immunostaining, and does not cross-react with lipophosphoglycan isolated from L. (V.) braziliensis promastigotes. An indirect immunofluorescence study indicated that the antigenic GLs are present at the L. (V.) braziliensis promastigote surface. SST-1 reacted with promastigotes of L. (V.) naiffi and L. (V.) guyanensis, but not with species in the L. Leishmania subgenus i.e. L. (L.) amazonensis, L. (L.) chagasi, or L. (L.) major. All L. (V.) braziliensis serodemes tested were reactive with SST-1. These results indicate that SST-1 recognizes specific GLs expressed by species of the Viannia subgenus, and will be particularly useful for identification of L. (V.) braziliensis.

Keywords

Leishmania (Viannia) braziliensismonoclonal antibodyglycolipidpromastigotes
Type
Research Article
Information
Parasitology , Volume 131 , Issue 6 , December 2005 , pp. 737 - 745
Copyright
© 2005 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ávila, J. L. and Rojas, M. ( 1990). A galactosyl(α1−3)mannose epitope on phospholipids of Leishmania mexicana and L. braziliensis is recognized by trypanosomatid-infected human sera. Journal of Clinical Microbiology 28, 1530–1537.Google Scholar
Ávila, J. L., Rojas, M. and Galili, U. ( 1989). Immunogenic Galα1−3Gal carbohydrate epitopes are present on pathogenic american Trypanosoma and Leishmania. Journal of Immunology 142, 2828–2834.Google Scholar
Bradford, M. M. ( 1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle Scholar
Dittmer, J. C. and Lester, R. L. ( 1964). A simple, specific spray for the detection of phospholipids on thin layer chromatography. Journal of Lipid Research 5, 126–127.Google Scholar
Dubois, M., Gilles, K., Halmilton, J. K., Rebers, P. A. and Smith F. ( 1956). Colorimetric method for determination of sugar and related substances. Analytical Chemistry 28, 350–356.CrossRefGoogle Scholar
Giorgio, S., Santos, M. R. M., Straus, A. H., Takahashi, H. K. and Barbieri, C. L. ( 2003). Effect of glycosphingolipids purified from Leishmania (L.) amazonensis amastigotes on human peripheral lymphocytes. Clinical Diagnostic Laboratory Immunology 10, 469–472.Google Scholar
Guha-Niyogi, A., Sullivan, D. R. and Turco, S. J. ( 2001). Glycoconjugate structures of parasitic protozoa. Glycobiology 11, 45R–59R.CrossRefGoogle Scholar
Handman, E. ( 1999). Cell biology of Leishmania. Advances in Parasitology 44, 1–39.CrossRefGoogle Scholar
Herwaldt, B. L. ( 1999). Leishmaniasis. Lancet 354, 1191–1199.CrossRefGoogle Scholar
Lainson, R. and Shaw, J. J. ( 1987). Evolution, classification and geographical distribution. In Leishmaniasis in Biology and Medicine ( ed. Peters, W. E. and Killick-Kendrick, R.), pp. 1–120. Academic Press, London.
McConville, M. J. and Blackwell, J. M. ( 1991). Developmental changes in the glycosylated phosphatidylinositols of the Leishmania donovani. Journal of Biological Chemistry 266, 15170–15179.Google Scholar
McConville, M. J. and Ferguson, M. A. J. ( 1993). The structure, biosynthesis and function of glycosylated phosphatidylinositols in parasitic protozoa and higher eukaryotes. The Biochemical Journal 294, 305–324.CrossRefGoogle Scholar
McNeely, T. B., Rosen, G., Londner, M. V. and Turco, S. J. ( 1989). Inhibitory effects on protein kinase C by lipophosphoglycan fragments and glycosylphosphatidyl inositol antigens on the protozoan parasite Leishmania. The Biochemical Journal 259, 601–604.CrossRefGoogle Scholar
Muskus, C., Segura, I., Oddone, R., Turco, S. J., Leiby, D. A., Toro, L., Robledo, S. and Saravia, N. G. ( 1997). Carbohydrate and LPG expression in Leishmania Viannia subgenus. Journal of Parasitology 83, 671–678.CrossRefGoogle Scholar
Ritting, M. G. and Bogdan, C. ( 2000). Leishmania-host-cell interaction: complexities and alternative views. Parasitology Today 16, 292–297.CrossRefGoogle Scholar
Shaw, J. J., Lainson, R., McMahon-Pratt, D. and David, J. R. ( 1986). Serodemes of the Leishmania braziliensis complex. In Leishmania Taxonomie et Phylogenèse. Applications Éco-épidémiologiques ( ed. Rioux, J.), pp. 179–183. Colloque International, 2–6 July, 1984, Montpellier, IMEE.
Silveira, T. G. V., Suzuki, E., Takahashi, H. K. and Straus, A. H. ( 2001). Inhibition of macrophage invasion by monoclonal antibodies specific to Leishmania (Viannia) braziliensis promastigotes and characterisation of their antigens. International Journal for Parasitology 31, 1451–1458.CrossRefGoogle Scholar
Silveira, T. G. V., Takahashi, H. K. and Straus, A. H. ( 2003). Immunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3 and SST-4. Parasitology 127, 1–8.CrossRefGoogle Scholar
Soares , R. P. P., Cardoso, T. L., Barron, T., Araujo, M. S. S., Pimenta, P. F. P. and Turco, S. J. ( 2005). Leishmania braziliensis: a novel mechanism in the lipophosphoglycan regulation during metacyclogenesis. International Journal for Parasitology 35, 245–253.CrossRefGoogle Scholar
Straus, A. H., Levery, S. B., Jasiulionis, M. G., Salyan, M. E. K., Steele, S. J., Travassos, L. R., Hakomori, S. and Takahashi, H. K. ( 1993). Stage-specific glycosphingolipids from amastigote forms of Leishmania (L.) amazonensis. Journal of Biological Chemistry 268, 13723–13730.Google Scholar
Straus, A. H., Valero, V. B., Takizawa, C. M., Levery, S. B., Toledo, M. S., Suzuki, E., Salyan, M. E. K, Hakomori, S., Barbiéri, C. L. and Takahashi, H. K. ( 1997). Glycospingolipid antigens from Leishmania (L.) amazonensis amastigotes. Binding of anti-glycosphingolipid monoclonal antibodies in vitro and in vivo. Brazilian Journal of Medical and Biological Research 30, 395–389.Google Scholar
Suzuki, E., Takahashi, H. K. and Straus, A. H. ( 2002). Role of beta-D-galactofuranose in Leishmania major macrophage invasion. Infection and Immunity 70, 6592–6596.CrossRefGoogle Scholar
Suzuki, E., Toledo, M. S., Takahashi, H. K. and Straus, A. H. ( 1997). A monoclonal antibody directed to terminal residue of β-galactofuranose of a glycolipid antigen isolated from Paracoccidioides brasiliensis: cross-reactivity with Leishmania major and Trypanosoma cruzi. Glycobiology 7, 463–468.CrossRefGoogle Scholar
Tachado, S. D., Mazhaari-Tabiri, R. and Schofield, L. ( 1999). Specificity in signal transdution among glycosylphosphatidylinositols of Plasmodium falciparum, Trypanosoma brucei, Trypanosoma cruzi and Leishmania ssp. Parasite Immunology 21, 609–617.CrossRefGoogle Scholar
Takahashi, H. K., Metoki, R. and Hakomori, S. ( 1988). Immunoglobulin G3 monoclonal antibody directed to Tn antigen (Tumor-associated α-N-acetylgalactosaminyl epitope) that does cross-react with blood group A antigen. Cancer Research 48, 4361–4367.Google Scholar
Toledo, M. S., Suzuki, E., Straus, A. H. and Takahashi, H. K. ( 1995). Glycolipids from Paracoccidioides brasiliensis. Isolation of a galactofuranose-containing glycolipid reactive with sera of patients with paracoccidioidomycosis. Journal of Medical and Veterinary Mycology 33, 247–251.Google Scholar
Zawadzki, J., Scholz, C., Currie, G., Coombs, G. H. and McConville, M. J. ( 1998). The glycoinositolphospholipids from Leishmania panamensis contain unsual glycan and lipid moieties. Journal of Molecular Biology 282, 287–299.CrossRefGoogle Scholar