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Ion channels of Fasciola hepatica incorporated into planar lipid bilayers

Published online by Cambridge University Press:  19 January 2004

J. H. JANG ,
S. D. KIM ,
J. B. PARK ,
S. J. HONG  and
P. D. RYU
J. H. JANG
Affiliation:
Laboratory of Pharmacology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1 Sillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
S. D. KIM
Affiliation:
Laboratory of Pharmacology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1 Sillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
J. B. PARK
Affiliation:
Department of Physiology, Chungnam National University College of Medicine, Taejeon 130-131, Republic of Korea
S. J. HONG
Affiliation:
Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Republic of Korea
P. D. RYU
Affiliation:
Laboratory of Pharmacology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1 Sillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
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Abstract

Ion channels are important target sites of anthelmintics, but little is known about those in Fasciola hepatica. In this work, we applied a planar lipid bilayer technique to characterize the properties of single ion channels in F. hepatica. Under a 200/40 mM KCl gradient, a large conductance channel of 251 pS was observed in 18% of the membranes studied. The channel was selective to K+ over Cl− with a permeability ratio of K+ to Cl− (PK/PCl) of 4·9. Open state probability (Po) of the channel was less than 0·5 and dependent on voltage (−60~+40 mV) and Ca2+ (~100 μM). The other two types of single channels observed in 11 and 5% of membranes, respectively, were a K+-permeable channel of 80 pS (PK/PCl=4·6) and a Cl−-permeable channel of 64 pS (PK/PCl=0·058). Open state probability of both channels showed little voltage dependence. The results indicate that distinct single channels of 60~251 pS are present in relative abundance and, in addition, that the planar lipid bilayer technique can be a useful tool for the study of single ion channels in F. hepatica.

Keywords

ion channelsFasciola hepaticaplanar lipid bilayer
Type
Research Article
Information
Parasitology , Volume 128 , Issue 1 , January 2004 , pp. 83 - 89
Copyright
2004 Cambridge University Press

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