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Involvement of sulfated glycosaminoglycans on the development and attachment of Trypanosoma cruzi to the luminal midgut surface in the vector, Rhodnius prolixus

Published online by Cambridge University Press:  09 September 2011

MARCELO S. GONZALEZ ,
LUIZ-CLAUDIO F. SILVA ,
J. M. ALBUQUERQUE-CUNHA ,
NADIR F. S. NOGUEIRA ,
DÉBORA P. MATTOS ,
DANIELE P. CASTRO ,
PATRICIA AZAMBUJA  and
ELOI S. GARCIA
MARCELO S. GONZALEZ*
Affiliation:
Laboratório de Biologia de Insetos, Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense (UFF), Morro do Valonguinho s/n – Centro –Niterói, RJ, 24001-970, Brazil Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq), Brazil
LUIZ-CLAUDIO F. SILVA
Affiliation:
Programa de Glicobiologia, Instituto de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, CEP 21941-590, Brazil Laboratório de Interações Celulares, Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-590, Brazil
J. M. ALBUQUERQUE-CUNHA
Affiliation:
Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil
NADIR F. S. NOGUEIRA
Affiliation:
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Horto, Campos dos Goytacases, RJ, 28015-620, Brazil
DÉBORA P. MATTOS
Affiliation:
Laboratório de Biologia de Insetos, Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense (UFF), Morro do Valonguinho s/n – Centro –Niterói, RJ, 24001-970, Brazil
DANIELE P. CASTRO
Affiliation:
Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq), Brazil
PATRICIA AZAMBUJA
Affiliation:
Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq), Brazil
ELOI S. GARCIA
Affiliation:
Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21045-900, Brazil Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq), Brazil
*
*Corresponding author: Departamento de Biologia Geral, Instituto de Biologia, Universidade Federal Fluminense, Morro do Valonguinho s/n° Centro –Niterói, RJ, 24001-970, Brazil. Tel: +55 2126292285. Fax: +55 2126292376. E-mail: [email protected]
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Summary

In the present study, we investigated the involvement of sulfated glycosaminoglycans in both the in vivo development and adhesion of T. cruzi epimastigotes to the luminal surface of the digestive tract of the insect vector, Rhodnius prolixus. Pre-incubation of T. cruzi, Dm 28c epimastigotes with heparin, chondroitin 4-sulfate, chondroitin 6-sulfate or protamine chloridrate inhibited in vitro attachment of parasites to the insect midgut. Enzymatic removal of heparan sulfate moieties by heparinase I or of chondroitin sulfate moieties by chondroitinase AC from the insect posterior midgut abolished epimastigote attachment in vitro. These treatments also reduced the labelling of anionic sites exposed at the luminal surface of the perimicrovillar membranes in the triatomine midgut epithelial cells. Inclusion of chondroitin 4-sulfate or chondroitin 6-sulfate and to a lesser extent, heparin, in the T. cruzi-infected bloodmeal inhibited the establishment of parasites in R. prolixus. These observations indicate that sulfated glycosaminoglycans are one of the determinants for both adhesion of the T. cruzi epimastigotes to the posterior midgut epithelial cells of the triatomine and the parasite infection in the insect vector, R. prolixus.

Keywords

Trypanosoma cruziRhodnius prolixustriatomid-parasite interactionposterior midgutperitrophic and perimicrovillar membraneglycosaminoglycans
Type
Research Article
Information
Parasitology , Volume 138 , Issue 14 , December 2011 , pp. 1870 - 1877
Copyright
Copyright © Cambridge University Press 2011

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